anti-Mouse, Rat Lamin A Antibody | anti-LMNA antibody

Lamin A (Cleaved-Asp230) Antibody

Cat. Num. AAA853553

• Gene Names: LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Lamin A; Lamin A (Cleaved-Asp230) Antibody; 70 kDa lamin; LAMA; LMN1; anti-LMNA antibody

• Reactivity: Mouse, Rat
• Specificity: Lamin A (cleaved-Asp230) antibody detects endogenous levels of fragment of activated Lamin A resulting from cleavage adjacent to Asp230.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 574

• Applicable Applications for anti-LMNA antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from human Lamin A.
• All Sites: Human: Asp230; Mouse: Asp230; Rat: Asp229
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Cl-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Lamin A (cleaved-Asp230) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, treated with etoposide (25muM, 1hour), using Lamin A (cleaved-Asp230) antibody.)

References

Revekka L. Boguslavsky, Hum. Mol. Genet., Feb 2006; 15: 653-663. Dan Constantinescu, Stem Cells, Jan 2006; 24: 177-185. Richard L. Frock, Genes & Dev., Feb 2006; 20: 486-500. Jan Lammerding, J. Biol. Chem., Sep 2006; 281: 25768-25780.

NCBI and Uniprot Product Information

• NCBI GI #: 383792150
• NCBI GeneID: 4000
• NCBI Accession #: NP_001244303.1
• NCBI GenBank Nucleotide #: NM_001257374.2
• UniProt Accession #: P02545; P02546; Q5I6Y4; Q5I6Y6; Q5TCJ2; Q5TCJ3; Q6UYC3; Q969I8; B4DI32; D3DVB0; D6RAQ3; E7EUI9
• Molecular Weight: 69,249 Da
• NCBI Official Full Name: lamin isoform D
• NCBI Official Synonym Full Names: lamin A/C
• NCBI Official Symbol: LMNA
• NCBI Official Synonym Symbols: FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
• NCBI Protein Information: lamin
• UniProt Protein Name: Prelamin-A/C
• Protein Family: Lamin
• UniProt Gene Name: LMNA
• UniProt Synonym Gene Names: LMN1

NCBI Description

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

Uniprot Description

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:22431096, PubMed:10814726, PubMed:11799477, PubMed:18551513). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:18611980, PubMed:23666920).