Human Prelamin-A/C ELISA Kit | LMNA elisa kit

Human Prelamin-A/C ELISA Kit

Cat. Num. AAA2887770

• Gene Names: Lmna; Dhe
• Reactivity: Human
• Synonyms: Prelamin-A/C; Human Prelamin-A/C ELISA Kit; LMNA; LMN1; LMNA elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Prelamin-A/C
• Sequence Length: 665

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 78-5000 pg/mL
• Sensitivity: 40.8pg/mL
• Intra-assay Precision: Intra-Assay CV: <=7.1%
• Inter-assay Precision: Inter-Assay CV: <=9.7%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LMNA elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 162287370
• NCBI GeneID: 16905
• NCBI Accession #: NP_001002011.2
• NCBI GenBank Nucleotide #: NM_001002011.3
• Molecular Weight: 52,652 Da
• NCBI Official Full Name: prelamin-A/C isoform A
• NCBI Official Synonym Full Names: lamin A
• NCBI Official Symbol: Lmna
• NCBI Official Synonym Symbols: Dhe
• NCBI Protein Information: prelamin-A/C
• UniProt Protein Name: Prelamin-A/C
• Protein Family: Prelamin
• UniProt Gene Name: Lmna
• UniProt Synonym Gene Names: Lmn1
• UniProt Entry Name: LMNA_MOUSE

NCBI Description

This gene encodes a protein that is a member of the lamin family. Nuclear lamins, intermediate filament-like proteins, are the major components of the nuclear lamina, a protein meshwork associated with the inner nuclear membrane. This meshwork is thought to maintain the integrity of the nuclear envelope, participate in chromatin organization, and regulate gene transcription. Vertebrate lamins consist of two types, A and B. This protein is an A-type and is proposed to be developmentally regulated. In mouse deficiency of this gene is associated with muscular dystrophy. Mouse lines with different mutations in this gene serve as pathophysiological models for several human laminopathies. In humans, mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, May 2013]

Uniprot Description

lamin A/C: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. Cleaved by caspase-6 during apoptosis into a 40-45 kDa and a28 kDa fragment.

Protein type: Cytoskeletal

Cellular Component: cytoplasm; lamin filament; nuclear envelope; nuclear matrix; nuclear membrane; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function: protein binding; protein phosphatase 1 binding

Biological Process: establishment and/or maintenance of microtubule cytoskeleton polarity; muscle development; nuclear membrane organization and biogenesis; nuclear organization and biogenesis; positive regulation of osteoblast differentiation; regulation of cell migration; sterol regulatory element binding protein nuclear translocation; ventricular cardiac muscle cell development