Lipopolysaccharide-Induced TNF Factor Recombinant Protein | LITAF recombinant protein

Recombinant Human Lipopolysaccharide-Induced TNF Factor

Cat. Num. AAA146519

• Gene Names: LITAF; PIG7; SIMPLE; TP53I7
• Purity: Greater than 85% as determined by SDS-PAGE.
• Synonyms: Lipopolysaccharide-Induced TNF Factor; Recombinant Human Lipopolysaccharide-Induced TNF Factor; LITAF Human; Lipopolysaccharide-Induced TNF Factor Human Recombinant; Lipopolysaccharide-induced TNF-alpha factor; PIG7; SIMPLE; Lipopolysaccharide-induced tumor necrosis factor-alpha factor; LPS-induced TNF-alpha factor; p53-induced gene 7 protein; Small integral membrane protein of lysosome/late endosome; LITAF; LITAF recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85% as determined by SDS-PAGE.
• Form/Format: The LITAF solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MSVPGPYQAA TGPSSAPSAP PSYEETVAVN SYYPTPPAPM PGPTTGLVTG PDGKGMNPPS YYTQPAPIPN NNPITVQTVY VQHPITFLDR PIQMCCPSCN KMIVSQLSYN AGALTWLSCG SLCLLGCIAG CCFIPFCVDA LQDVDHYCPN CRALLGTYKR L.
• Sequence Length: 161

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for LITAF recombinant protein

Description: LITAF Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 181 amino acids (1-161) and having a molecular mass of 19.2 kDa. LITAF is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Lipopolysaccharide-induced TNF-alpha factor (LITAF) is a small integral membrane protein of lysosome/late endosome. The expression of inflammatory cytokines such as TNF-alpha in Lipopolysaccharide-induced processes is mediated by LITAF. LITAF connects to STAT6B, which belongs to the STAT6 family forming a complex on the TNF-alpha promoter that modifies TNF activity. High levels of expression of LITAF mRNA are observed mostly in the placenta, peripheral blood leukocytes, lymph nodes and spleen.

Product Categories/Family for LITAF recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 210147505
• NCBI GeneID: 9516
• NCBI Accession #: NP_001129944.1
• NCBI GenBank Nucleotide #: NM_001136472.1
• UniProt Accession #: Q99732; Q05DW0; Q9C0L6; D3DUG1; G5E9K0
• Molecular Weight: 15,824 Da
• NCBI Official Full Name: lipopolysaccharide-induced tumor necrosis factor-alpha factor isoform 1
• NCBI Official Synonym Full Names: lipopolysaccharide-induced TNF factor
• NCBI Official Symbol: LITAF
• NCBI Official Synonym Symbols: PIG7; SIMPLE; TP53I7
• NCBI Protein Information: lipopolysaccharide-induced tumor necrosis factor-alpha factor; LPS-induced TNF-alpha factor; lipopolysaccharide-induced TNF-alpha factor; p53-induced gene 7 protein; small integral membrane protein of lysosome/late endosome; tumor protein p53 inducible protein 7
• UniProt Protein Name: Lipopolysaccharide-induced tumor necrosis factor-alpha factor
• Protein Family: Lipopolysaccharide-induced tumor necrosis factor-alpha factor
• UniProt Gene Name: LITAF
• UniProt Synonym Gene Names: PIG7; SIMPLE; LPS-induced TNF-alpha factor
• UniProt Entry Name: LITAF_HUMAN

NCBI Description

Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppressor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

LITAF: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF- alpha) gene expression. Interacts with NEDD4. Interacts with WWOX. Isoform 2 may interact with STAT6. By bacterial lipopolysaccharides (LPS) or p53/TP53. In monocytes by the Bacillus Calmette-Guerin (BCG). Ubiquitously and abundantly expressed. Expressed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 16p13.13

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; lysosomal membrane; cytoplasm; plasma membrane

Molecular Function: protein binding; signal transducer activity; WW domain binding

Biological Process: regulation of transcription from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; apoptosis; negative regulation of NF-kappaB import into nucleus; signal transduction; aging; regulation of cytokine production

Disease: Charcot-marie-tooth Disease, Demyelinating, Type 1c