Human Lipase H ELISA Kit | LIPH elisa kit

Human Lipase H ELISA Kit

Cat. Num. AAA761104

• Gene Names: LIPH; AH; LAH2; ARWH2; HYPT7; LPDLR; PLA1B; mPA-PLA1
• Reactivity: Human
• Synonyms: Lipase H; Human Lipase H ELISA Kit; LIPH/Phospholipase A1 member B/LPD lipase-related protein/Membrane-associated phosphatidic acid-selective phospholipase A1-alpha (mPA-PLA1 alpha)/AH/ARWH2/LAH2/LPDLR/PLA1B/mPA-PLA1/LPDLR/MPAPLA1/PLA1B; LIPH elisa kit

• Reactivity: Human
• Sequence Length: 451

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LIPH elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human LIPH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LIPH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LIPH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human LIPH can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 22773576
• NCBI GeneID: 200879
• Molecular Weight: 50,859 Da
• NCBI Official Full Name: lipase H
• NCBI Official Synonym Full Names: lipase H
• NCBI Official Symbol: LIPH
• NCBI Official Synonym Symbols: AH; LAH2; ARWH2; HYPT7; LPDLR; PLA1B; mPA-PLA1
• NCBI Protein Information: lipase member H
• UniProt Protein Name: Lipase member H
• Protein Family: Lipase
• UniProt Gene Name: LIPH
• UniProt Synonym Gene Names: LPDLR; MPAPLA1; PLA1B; LIPH; mPA-PLA1 alpha
• UniProt Entry Name: LIPH_HUMAN

NCBI Description

This gene encodes a membrane-bound member of the mammalian triglyceride lipase family. It catalyzes the production of 2-acyl lysophosphatidic acid (LPA), which is a lipid mediator with diverse biological properties that include platelet aggregation, smooth muscle contraction, and stimulation of cell proliferation and motility. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPH: Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG). Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7); also known as alopecia universalis congenita Mari type. A condition characterized by the presence of less than the normal amount of hair. Affected individuals have sparse or absent scalp, axillary and body hair and sparse eyebrows and eyelashes. Defects in LIPH are the cause of woolly hair autosomal recessive type 2 (ARWH2). A hair shaft disorder characterized by fine and tightly curled hair. Compared to normal curly hair that is observed in some populations, woolly hair grows slowly and stops growing after a few inches. Under light microscopy, woolly hair shows some structural anomalies, including trichorrhexis nodosa and tapered ends. Some individuals may present with hypotrichosis. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Secreted, signal peptide; Phospholipase; Secreted; EC 3.1.1.-

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space; plasma membrane

Molecular Function: heparin binding; phospholipase activity

Biological Process: lipid catabolic process; lipid digestion

Disease: Hypotrichosis 7