Human Leukocyte immunoglobulin-like receptor subfamily B member 1 ELISA Kit | LILRB1 elisa kit

Human Leukocyte immunoglobulin-like receptor subfamily B member 1 ELISA Kit

Cat. Num. AAA765790

• Gene Names: LILRB1; ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• Reactivity: Human
• Synonyms: Leukocyte immunoglobulin-like receptor subfamily B member 1; Human Leukocyte immunoglobulin-like receptor subfamily B member 1 ELISA Kit; LILRB1/Monocyte/macrophage immunoglobulin-like receptor 7 (MIR-7)/CD85 antigen-like family member J/Immunoglobulin-like transcript 2 (ILT-2)/LIR-1/Leukocyte immunoglobulin-like receptor 1/CD85j/ILT2/LIR1/MIR7/CD85 antigen-like family member J/CD85j/CD85j antigen/Ig-like transcript 2/ILT-2/ILT2FLJ37515/Immunoglobulin-like transcript 2/leukocyte Ig-like receptor-1/Leukocyte immunoglobulin-like receptor 1/leukocyte immunoglobulin-like receptor subfamily B member 1/leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform/leukocyte immunoglobulin-like receptor; subfamily B (with TM and ITIM domains)/Monocyte/macrophage immunoglobulin-like receptor 7; LILRB1 elisa kit

• Reactivity: Human
• Sequence Length: 650

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LILRB1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology anti-Human LILRB1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LILRB1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LILRB1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human LILRB1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 612149789
• NCBI GeneID: 10859
• NCBI Accession #: NP_006660.4
• NCBI GenBank Nucleotide #: NM_006669.6
• Molecular Weight: 49,356 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 1 isoform 1
• NCBI Official Synonym Full Names: leukocyte immunoglobulin like receptor B1
• NCBI Official Symbol: LILRB1
• NCBI Official Synonym Symbols: ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 1
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 1
• Protein Family: Leukocyte immunoglobulin-like receptor subfamily
• UniProt Gene Name: LILRB1
• UniProt Synonym Gene Names: ILT2; LIR1; MIR7; LIR-1; Leukocyte immunoglobulin-like receptor 1; ILT-2; MIR-7
• UniProt Entry Name: LIRB1_HUMAN

NCBI Description

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LILRB1: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: cytoplasm; external side of plasma membrane; plasma membrane

Molecular Function: HLA-A specific inhibitory MHC class I receptor activity; HLA-B specific inhibitory MHC class I receptor activity; MHC class I protein binding; MHC class I receptor activity; protein homodimerization activity; protein phosphatase 1 binding; SH2 domain binding

Biological Process: defense response to virus; Fc receptor mediated inhibitory signaling pathway; interferon-gamma production; negative regulation of alpha-beta T cell activation; negative regulation of calcium ion transport; negative regulation of cell cycle; negative regulation of cytokine secretion during immune response; negative regulation of endocytosis; negative regulation of interferon-gamma biosynthetic process; negative regulation of interferon-gamma production; negative regulation of mononuclear cell proliferation; negative regulation of natural killer cell mediated cytotoxicity; negative regulation of serotonin secretion; negative regulation of T cell mediated cytotoxicity; negative regulation of T cell proliferation; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of apoptosis; positive regulation of cytolysis; positive regulation of defense response to virus by host; receptor internalization; regulation of immune response; response to virus; signal transduction; T cell proliferation during immune response