Leukemia Inhibitory Factor Recombinant Protein | LIF recombinant protein

Mouse Leukemia Inhibitory Factor Recombinant

Cat. Num. AAA553093

• Purity: >98%, as determined by SDS-PAGE and HPLC.
• Synonyms: Leukemia Inhibitory Factor; Mouse Leukemia Inhibitory Factor Recombinant; Leukemia Inhibitory Factor ; LIF; HILDA; mlpli; LIF recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC.
• Form/Format: Recombinant Leukemia Inhibitory Factor was lyophilized from a 0.2 um filtered PBS pH 7.5.
• Sequence: MKVLAAGIVP LLLLVLHWKH GAGSPLPITP VNATCAIRHP CHGNLMNQIK NQLAQLNGSA NALFISYYTA QGEPFPNNVE KLCAPNMTDF PSFHGNGTEK TKLVELYRMV AYLSASLTNI TRDQKVLNPT AVSLQVKLNA TIDVMRGLLS NVLCRLCNKY RVGHVDVPPV PDHSDKEAFQ RKKLGCQLLG TYKQVISVVV QAF
• Sequence Length: 203

• Domain: 4 helix cytokine core
• Host Note: Optimized DNA sequence encoding Mouse LIF mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Molecular Weight Note: Native Mouse LIF is generated by the proteolytic removal of the signal peptide; and propeptide, the molecule has a calculated molecular mass of approximately 20 kDa.; ; LIF is a disulfide-linked monomer protein consisting of 181 amino acid residue subunits, and migrates as an approximately 20 kDa protein under non-reducing; and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for LIF recombinant protein

LIF, a multifunctional, secreted glycoprotein that exists in both soluble and matrix-bound forms, displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation (2). It is now clear that LIF is related in both structure and mechanism of action to the interleukin (IL) -6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1 (2). The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130

Product Categories/Family for LIF recombinant protein

Cytokines

References

• arcuate nucleus proopiomelanocortin neurons mediate the acute anorectic actions of Leukemia Inhibitory Factor via gp130 .Endocrinology, Feb 2010; 151: 606 - 616.
• neuroprotective and axon growth-promoting effects following inflammatory stimulation on mature retinal ganglion cells in mice depend on ciliary neurotrophic factor and Leukemia Inhibitory Factor .J. Neurosci., Nov 2009; 29: 14334 - 14341.
• socs1, socs3, and pias1 promote myogenic differentiation by inhibiting the Leukemia Inhibitory Factor-induced jak1/stat1/stat3 pathway .Mol. Cell. Biol., Sep 2009; 29: 5084 - 5093.
• interleukin-11 and Leukemia Inhibitory Factor regulate the adhesion of endometrial epithelial cells: implications in fertility regulation .Endocrinology, Jun 2009; 150: 2915 - 2923.
• Leukemia Inhibitory Factor controls a retinal rescue mechanism which supports survival of photoreceptor cells in vivo .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 3880.