Proto-oncogene tyrosine-protein kinase LCK (Lck) Recombinant Protein | Lck recombinant protein

Recombinant Mouse Proto-oncogene tyrosine-protein kinase LCK (Lck)

Cat. Num. AAA950936

• Gene Names: Lck; Lsk; Lskt; Hck-3; p56Lck; p56<lck>
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Proto-oncogene tyrosine-protein kinase LCK (Lck); Recombinant Mouse Proto-oncogene tyrosine-protein kinase LCK (Lck); Lck recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-509, Full length protein
• Sequence: GCVCSSNPEDDWMENIDVCENCHYPIVPLDSKISLPIRNGSEVRDPLVTYEGSLPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHDLVRHYTNASDGLCTKLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHPRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLNVNKLLDMAAQIAEGMAFIEEQNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYHLMMLCWKERPEDRPTFDYLRSVLDDFFTATEGQYQPQP
• Sequence Length: 508

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Lck recombinant protein

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described.

NCBI and Uniprot Product Information

• NCBI GI #: 244792312
• NCBI GeneID: 16818
• NCBI Accession #: NP_001155905.1
• NCBI GenBank Nucleotide #: NM_001162433.1
• UniProt Accession #: P06240; Q61794; Q61795; Q62320; Q91X65
• Molecular Weight: 57,943 Da
• NCBI Official Full Name: proto-oncogene tyrosine-protein kinase LCK isoform b
• NCBI Official Synonym Full Names: lymphocyte protein tyrosine kinase
• NCBI Official Symbol: Lck
• NCBI Official Synonym Symbols: Lsk; Lskt; Hck-3; p56Lck; p56<lck>
• NCBI Protein Information: proto-oncogene tyrosine-protein kinase LCK
• UniProt Protein Name: Proto-oncogene tyrosine-protein kinase LCK
• Protein Family: Proto-oncogene tyrosine-protein kinase
• UniProt Gene Name: Lck
• UniProt Synonym Gene Names: Lsk-t; LSK

Uniprot Description

Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP (). Interacts with UNC119; this interaction plays a crucial role in activation of LCK ().