Human La-related protein 7 ELISA Kit | LARP7 elisa kit

Human La-related protein 7 ELISA Kit

Cat. Num. AAA760637

• Gene Names: LARP7; ALAZS; PIP7S; HDCMA18P
• Reactivity: Human
• Synonyms: La-related protein 7; Human La-related protein 7 ELISA Kit; LARP7/La-related protein 7/La ribonucleoprotein domain family member 7/P-TEFb-interaction protein for 7SK stability/PIP7S/HDCMA18P; LARP7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LARP7. No significant cross-reactivity or interference between LARP7 and analogues was observed.
• Sequence Length: 582

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LARP7 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 109809741
• NCBI GeneID: 51574
• NCBI Accession #: NP_057732.2
• NCBI GenBank Nucleotide #: NM_016648.3
• Molecular Weight: 67,654 Da
• NCBI Official Full Name: la-related protein 7 isoform 1
• NCBI Official Synonym Full Names: La ribonucleoprotein domain family member 7
• NCBI Official Symbol: LARP7
• NCBI Official Synonym Symbols: ALAZS; PIP7S; HDCMA18P
• NCBI Protein Information: la-related protein 7
• UniProt Protein Name: La-related protein 7
• Protein Family: La-related protein
• UniProt Gene Name: LARP7
• UniProt Synonym Gene Names: PIP7S
• UniProt Entry Name: LARP7_HUMAN

NCBI Description

This gene encodes a protein which is found in the 7SK snRNP (small nuclear ribonucleoprotein). This snRNP complex inhibits a cyclin-dependent kinase, positive transcription elongation factor b, which is required for paused RNA polymerase II at a promoter to begin transcription elongation. A pseudogene of this gene is located on chromosome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

Uniprot Description

LARP7: Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: cytoplasm; Golgi apparatus; nucleoplasm; nucleus; ribonucleoprotein complex

Molecular Function: nucleotide binding; protein binding

Biological Process: RNA processing

Disease: Alazami Syndrome