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L-amino-acid oxidase Recombinant Protein | LOC100533346 recombinant protein

Recombinant Aplysia californica L-amino-acid oxidase, partial

Gene Names
LOC100533346; LAO; LAAO; Escapin
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
L-amino-acid oxidase; Recombinant Aplysia californica L-amino-acid oxidase; partial; LOC100533346 recombinant protein
Ordering
For Research Use Only!
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
49-529. Partial.
Sequence
ANSAYMLRESGLDIAVFEYSDRVGGRLFTYQLPNTPDVNLEIGGMRFIEGAMHRLWKVISELGLTPKVFKEGFGKEGRQRFYLRGQSLTKKQVKSGDVPYDLSPEEKANQGRLVEYYLEKLTGLQLNGGPLKREVALKLTVPDGRFLYDLTFDEALDLVASPEGKEFARDTHVFTSEVTLDASAISIFDDHLGEDYYGSEIYTLEEGMSSVPQGLLQTFLDAAKSNEFFPNNHLKALRRRTNGQYVLYFEPTTSKDGQTTINYLEPLKVVCAQRVILAMPVYALRQLDWSQLRNDRATQAYRAVRPMPASKVFMTFDQPWWLQNERKSWVTKSDALFSQMYDWQKSEASGDYILIASYADGLKAQYLRELKNQGEDIPGSDPGYNQVTVPLKDAILEHLTEAYGVERDSIPEPVTAASQFWTDYPFGCGWITWRAGYHFDDVISTMRRPSLKDEVYVVGSDYSWGLISSWIEGALETSENV
Sequence Length
529
Species
Aplysia californica (California sea hare)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60,300 Da
NCBI Official Full Name
L-amino-acid oxidase
NCBI Official Symbol
LOC100533346
NCBI Official Synonym Symbols
LAO; LAAO; Escapin
NCBI Protein Information
L-amino-acid oxidase
UniProt Protein Name
L-amino-acid oxidase
Protein Family
UniProt Gene Name
LAO
UniProt Synonym Gene Names
LAO

Uniprot Description

Catalyzes the oxidative deamination of positively charged L-amino acids L-Lys and L-Arg and, much less efficiently, of amino acids L-His and L-Tyr. Has antimicrobial activity. Inhibits growth of Gram-negative bacteria E.coli strain MC4100 (MIC=0.62 µg/ml), E.coli strain C921-b2, S.typhimurium strain AA 140 (MIC=0.62 µg/ml), P.aeruginosa strain PAO1 (MIC=0.31 µg/ml) and V.harveyi strain BB170 (MIC=0.25 µg/ml). Inhibits growth of Gram-positive bacteria S.aureus strain 6835 (MIC=0.31 µg/ml), S.pyogenes strain NZ131 (MIC=0.62 µg/ml), B.subtilis strain 168 (MIC=2.50 µg/ml) and B.subtilis strain WB600 (MIC=2.50 µg/ml). The antibacterial effect can be bacteriostatic, mediated mostly by H2O2, as well as bactericidal. The latter appears to be due to unstable intermediates generated from alpha-keto-epsilon-aminocaproic acid, an intermediate of escapin-deaminated L-Lys, and H2O2. The bactericidal effect is stronger at lower pH levels. Intermediates generated from L-Arg have no bactericidal effect. Inhibits growth of yeast species S.cerevisiae strain BY4761 (MIC=5.0 µg/ml) and C.krusei (MIC=5.0 µg/ml). Inhibits growth of C.sphaerospermum in a lysine-dependent manner. As components of ink, products from escapin's enzymatic activity may play a role in deterring eukaryotic predators.

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Product Notes

The LOC100533346 lao (Catalog #AAA1291483) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 49-529. Partial. The amino acid sequence is listed below: ANSAYMLRES GLDIAVFEYS DRVGGRLFTY QLPNTPDVNL EIGGMRFIEG AMHRLWKVIS ELGLTPKVFK EGFGKEGRQR FYLRGQSLTK KQVKSGDVPY DLSPEEKANQ GRLVEYYLEK LTGLQLNGGP LKREVALKLT VPDGRFLYDL TFDEALDLVA SPEGKEFARD THVFTSEVTL DASAISIFDD HLGEDYYGSE IYTLEEGMSS VPQGLLQTFL DAAKSNEFFP NNHLKALRRR TNGQYVLYFE PTTSKDGQTT INYLEPLKVV CAQRVILAMP VYALRQLDWS QLRNDRATQA YRAVRPMPAS KVFMTFDQPW WLQNERKSWV TKSDALFSQM YDWQKSEASG DYILIASYAD GLKAQYLREL KNQGEDIPGS DPGYNQVTVP LKDAILEHLT EAYGVERDSI PEPVTAASQF WTDYPFGCGW ITWRAGYHFD DVISTMRRPS LKDEVYVVGS DYSWGLISSW IEGALETSEN V. It is sometimes possible for the material contained within the vial of "L-amino-acid oxidase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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