Ragulator complex protein LAMTOR1 (LAMTOR1) Recombinant Protein | LAMTOR1 recombinant protein

Recombinant Human Ragulator complex protein LAMTOR1 (LAMTOR1)

Cat. Num. AAA1443133

• Gene Names: LAMTOR1; p18; PDRO; C11orf59; p27RF-Rho; Ragulator1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Ragulator complex protein LAMTOR1 (LAMTOR1); Recombinant Human Ragulator complex protein LAMTOR1 (LAMTOR1); Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1; Lipid raft adaptor protein p18; Protein associated with DRMs and endosomes; p27Kip1-releasing factor from RhoA; p27RF-Rho; LAMTOR1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-161. Full Length of Mature Protein
• Sequence: GCCYSSENEDSDQDREERKLLLDPSSPPTKALNGAEPNYHSLPSARTDEQALLSSILAKTASNIIDVSAADSQGMEQHEYMDRARQYSTRLAVLSSSLTHWKKLPPLPSLTSQPHQVLASEPIPFSDLQQVSRIAAYAYSALSQIRVDAKEELVVQFGIP

• Species: Homo sapiens (Human)
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for LAMTOR1 recombinant protein

As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation.

Product Categories/Family for LAMTOR1 recombinant protein

Metabolism

References

"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 8923579
• NCBI GeneID: 55004
• NCBI Accession #: NP_060377.1
• NCBI GenBank Nucleotide #: NM_017907.2
• UniProt Accession #: Q6IAA8; Q8WZ09; Q9NWT0
• Molecular Weight: 21.6 kDa
• NCBI Official Full Name: ragulator complex protein LAMTOR1
• NCBI Official Synonym Full Names: late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
• NCBI Official Symbol: LAMTOR1
• NCBI Official Synonym Symbols: p18; PDRO; C11orf59; p27RF-Rho; Ragulator1
• NCBI Protein Information: ragulator complex protein LAMTOR1
• UniProt Protein Name: Ragulator complex protein LAMTOR1
• Protein Family: Ragulator complex protein
• UniProt Gene Name: LAMTOR1
• UniProt Synonym Gene Names: C11orf59; PDRO; p27RF-Rho
• UniProt Entry Name: LTOR1_HUMAN

Uniprot Description

LAMTOR1: Regulator of the TOR pathway, a signaling cascade that promotes cell growth in response to growth factors, energy levels, and amino acids. As part of the Ragulator complex, recruits the Rag GTPases and the mTORC1 complex to lysosomes, a key step in activation of the TOR signaling cascade by amino acids. Directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation. Part of the Ragulator complex composed of LAMTOR1, LAMTOR2 and LAMTOR3. Interacts with LAMTOR2 and LAMTOR3; the interaction is direct. The Ragulator complex interacts with both the mTORC1 complex and heterodimers of the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD. Interacts with RRAGB and RRAGD; the interaction is direct indicating that it probably constitutes the main RAG-interacting subunit of the Ragulator complex. Interacts with MMP14. Interacts with CDKN1B; prevents the interaction of CDKN1B with RHOA leaving RHOA in a form accessible to activation by ARHGEF2.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; late endosome membrane; lipid raft; lysosomal membrane; lysosome; plasma membrane

Molecular Function: guanyl-nucleotide exchange factor activity; protein binding; protein complex scaffold

Biological Process: cell cycle arrest; cell growth; cholesterol homeostasis; endosome localization; endosome organization and biogenesis; gene expression; insulin receptor signaling pathway; lysosome localization; lysosome organization and biogenesis; macroautophagy; positive regulation of GTPase activity; positive regulation of MAPKKK cascade; positive regulation of TOR signaling pathway; regulation of receptor recycling; transcription initiation from RNA polymerase II promoter