Mouse Lysosomal Associated Membrane Protein 1 ELISA Kit | LAMP1 elisa kit

Mouse LAMP1 (Lysosomal Associated Membrane Protein 1) ELISA Kit

Cat. Num. AAA2533492

• Gene Names: LAMP1; LAMPA; CD107a; LGP120
• Reactivity: Mouse
• Synonyms: Lysosomal Associated Membrane Protein 1; Mouse LAMP1 (Lysosomal Associated Membrane Protein 1) ELISA Kit; LAMP1 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinantMouseLAMP1. No significant cross-reactivity or interference between MouseLAMP1 and analogues was observed.
• Sequence Length: 417

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: Min: 46.875pg/mL ; Max: 5000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAMP1 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseLAMP1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LAMP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LAMP1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LAMP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LAMP1.You can calculate the concentration of LAMP1in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 112380628
• NCBI GeneID: 3916
• NCBI Accession #: NP_005552.3
• NCBI GenBank Nucleotide #: NM_005561.3
• Molecular Weight: 38,986 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 1
• NCBI Official Synonym Full Names: lysosomal associated membrane protein 1
• NCBI Official Symbol: LAMP1
• NCBI Official Synonym Symbols: LAMPA; CD107a; LGP120
• NCBI Protein Information: lysosome-associated membrane glycoprotein 1
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 1
• Protein Family: Lysosome-associated membrane glycoprotein
• UniProt Gene Name: LAMP1
• UniProt Synonym Gene Names: LAMP-1; Lysosome-associated membrane protein 1
• UniProt Entry Name: LAMP1_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

LAMP1: Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. Belongs to the LAMP family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: cell soma; cytoplasm; dendrite; endosome membrane; external side of plasma membrane; integral to plasma membrane; late endosome; lysosomal membrane; lysosome; melanosome; membrane; multivesicular body; perinuclear region of cytoplasm; plasma membrane; sarcolemma; synaptic vesicle

Molecular Function: enzyme binding; protein binding; protein domain specific binding; viral receptor activity

Biological Process: autophagic cell death; autophagy; entry of virus into host cell; induction of apoptosis by granzyme; positive regulation of natural killer cell degranulation; positive regulation of natural killer cell mediated cytotoxicity; protein stabilization