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Laminin Beta 1 (LAMb1) Recombinant Protein | LAMb1 recombinant protein

Recombinant Laminin Beta 1 (LAMb1)

Gene Names
Lamb1; C77966; C80098; C81607; Lamb-1; Lamb1-1; D130003D08Rik
Reactivity
Mouse
Applications
SDS-Page, Western Blot
Purity
>95%
Synonyms
Laminin Beta 1 (LAMb1); Recombinant Laminin Beta 1 (LAMb1); LAMb1 recombinant protein
Ordering
For Research Use Only!
Host
E. coli
Reactivity
Mouse
Purity/Purification
>95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
Concentration
Original concentration: 200 ug/mL (varies by lot)
Sequence Length
1786
Applicable Applications for LAMb1 recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB.

(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Gln1053~Lys1258
Tags
N-terminal His and GST Tag
Subcellular Location
Secreted, extracellular matrix
Traits
Freeze-dried powder
Predicted isoelectric point
5.8
Predicted Molecular Mass
52.3kDa
Accurate Molecular Mass
52kDa as determined by SDS-PAGE reducing conditions.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Laminin subunit beta-1
NCBI Official Synonym Full Names
laminin B1
NCBI Official Symbol
Lamb1
NCBI Official Synonym Symbols
C77966; C80098; C81607; Lamb-1; Lamb1-1; D130003D08Rik
NCBI Protein Information
laminin subunit beta-1; laminin B1 chain; laminin B1 subunit 1; laminin-1 subunit beta; laminin-2 subunit beta; laminin-6 subunit beta; laminin-8 subunit beta; laminin-10 subunit beta; laminin-12 subunit beta
UniProt Protein Name
Laminin subunit beta-1
Protein Family
UniProt Gene Name
Lamb1
UniProt Synonym Gene Names
Lamb-1; Lamb1-1
UniProt Entry Name
LAMB1_MOUSE

Uniprot Description

LAMB1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; laminin-8 complex; laminin-2 complex; laminin-1 complex; perinuclear region of cytoplasm; laminin-10 complex; extracellular region; basement membrane; basal lamina

Molecular Function: integrin binding; protein binding; enzyme binding; extracellular matrix structural constituent; glycosphingolipid binding

Biological Process: odontogenesis; cell migration; learning and/or memory; neuronal-glial interaction involved in cerebral cortex radial glia guided migration; negative regulation of cell adhesion; cell adhesion; neurite development; embryo implantation; positive regulation of cell migration

Research Articles on LAMb1

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Product Notes

The LAMb1 lamb1 (Catalog #AAA2009201) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Laminin Beta 1 (LAMb1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin Beta 1 (LAMb1) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the LAMb1 lamb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Laminin Beta 1 (LAMb1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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