LAMA3 / Laminin Subunit Alpha-3 Recombinant Protein | LAMA3 recombinant protein

Human LAMA3 / Laminin Subunit Alpha-3 Recombinant Protein

• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: LAMA3 / Laminin Subunit Alpha-3; Human LAMA3 / Laminin Subunit Alpha-3 Recombinant Protein; Laminin subunit alpha-3; Epiligrin 170 kDa subunit; E170; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha; LAMNA; LAMA3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 1724

• Applicable Applications for LAMA3 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-3 is a subunit of laminin-5 (laminin-332 or epiligrin/kalinin/nicein), laminin-6 (laminin-311 or K-laminin) and laminin-7 (laminin-321 or KS-laminin).
• Entry Function: Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 38045908
• NCBI Accession #: NP_000218.2
• NCBI GenBank Nucleotide #: NP_000218.2
• UniProt Accession #: Q16787
• NCBI Official Full Name: laminin subunit alpha-3 isoform 2
• UniProt Protein Name: Laminin subunit alpha-3
• Protein Family: Laminin
• UniProt Gene Name: LAMA3
• UniProt Synonym Gene Names: LAMNA; E170
• UniProt Entry Name: LAMA3_HUMAN

Uniprot Description

LAMA3: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS). LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: laminin-5 complex; laminin-1 complex; extracellular region; basement membrane

Molecular Function: structural molecule activity; receptor binding

Biological Process: regulation of cell adhesion; extracellular matrix disassembly; hemidesmosome assembly; epidermis development; extracellular matrix organization and biogenesis; regulation of embryonic development; cell adhesion; regulation of cell migration

Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type; Laryngoonychocutaneous Syndrome; Epidermolysis Bullosa, Junctional, Herlitz Type