Laccase-1 Recombinant Protein | Lac1 recombinant protein
Recombinant Laccase-1
NCBI and Uniprot Product Information
Uniprot Description
Function: Lignin degradation and detoxification of lignin-derived products. Demethylates eucalyptus hard wood lignin. Has high activity against the non-phenolic heterocyclic compound ABTS, and lower activity against the phenolic substrates syringic acid, caffeic acid, syringaldazine, vanillic acid, catechol and levodihydroxyphenylalanine. Ref.1
Catalytic activity: 4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O. Ref.1
Cofactor: Binds 4 copper ions per monomer. Ref.1 UniProtKB Q12718
Enzyme regulation: Strongly inhibited by sodium azide, sodium cyanide, Li+, Sn+, Hg2+, and the disulfide-reducing agents beta-mercaptoethanol, dithiothreitol and thioglycolic acid. Moderately inhibited by Mn2+ and Fe2+, inhibition by these metal ions is stronger at 0.1 mM than at 1 mM. Moderately inhibited by Cu2+. Ref.1
Subcellular location: Secreted Ref.1.
Miscellaneous: On the 2D-gel the determined pI of this protein is: 4, its MW is: 64 kDa. Ref.1
Sequence similarities: Belongs to the multicopper oxidase family.
Biophysicochemical propertiesKinetic parameters:KM=14 µM for ABTS Ref.1KM=0.8 µM for syringaldazine Ref.1pH dependence:Optimum pH is 3.0 with ABTS as substrate, activity decreases sharply as pH increases, and no activity is seen at pH 7.0. Optimum pH is 4.0 with syringic acid as substrate, 5.0 with caffeic acid, syringaldazine, vanillic acid or catechol as substrate, and 6.0 with levodihydroxyphenylalanine as substrate. Inactive after 3 hours incubation at pH 2.0, retains 20% of its activity after 24 hours at pH 3.0 and 90% of its activity after 24 hours at pH 9.0. Ref.1Temperature dependence:Optimum temperature is 60 degrees Celsius. Shows 50% of its maximal activity at 20 degrees Celsius. Has a half-life of 53 minutes at 50 degrees Celsius, 23 minutes at 60 degrees Celsius, and less than 2 minutes at 70 degrees Celsius. Ref.1
Similar Products
Product Notes
The Laccase-1 lac1 (Catalog #AAA1161398) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-23aa; full length protein. The amino acid sequence is listed below: GPSTDLVIGN KGFDGGIDSA ILR. It is sometimes possible for the material contained within the vial of "Laccase-1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
