RNA-directed RNA polymerase L (L) Recombinant Protein | L recombinant protein

Recombinant Measles virus RNA-directed RNA polymerase L (L), partial

Cat. Num. AAA1363875

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: RNA-directed RNA polymerase L (L); Recombinant Measles virus RNA-directed RNA polymerase L (L); partial; L recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 653-843. Partial
• Sequence: YETVSAFITTDLKKYCLNWRYETISLFAQRLNEIYGLPSFFQWLHKRLETSVLYVSDPHCPPDLDAHVPLCKVPNDQIFIKYPMGGIEGYCQKLWTISTIPYLYLAAYESGVRIASLVQGDNQTIAVTKRVPSTWPYNLKKREAARVTRDYFVILRQRLHDIGHHLKANETIVSSHFFVYSKGIYYDGLLV

• Species: Measles virus (strain Ichinose-B95a) (MeV) (Subacute sclerose panencephalitis virus)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 9626952
• NCBI GeneID: 1489802
• NCBI Accession #: NP_056924.1
• NCBI GenBank Nucleotide #: NC_001498.1
• UniProt Accession #: Q9WMB3
• Molecular Weight: 247,738 Da
• NCBI Official Full Name: large polymerase protein
• NCBI Official Symbol: L
• NCBI Protein Information: large subunit of RNA dependent RNA polymerase (RdRp); RNA synthesis, capping, and polyadenylation
• UniProt Protein Name: RNA-directed RNA polymerase L
• UniProt Gene Name: L
• UniProt Synonym Gene Names: Protein L

Uniprot Description

RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.