KSR Blocking Peptide | KSR1 blocking peptide

KSR Antibody (N-term E174) Blocking peptide

• Gene Names: KSR1; KSR; RSU2
• Synonyms: KSR; KSR Antibody (N-term E174) Blocking peptide; Kinase suppressor of Ras 1; KSR1; KSR1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human KSR1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 923

• Cellular Location: Cytoplasm. Membrane; Peripheral membrane protein Endoplasmic reticulum membrane Note: In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for KSR1 blocking peptide

Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 677286751
• NCBI GeneID: 8844
• UniProt Accession #: Q8IVT5; Q13476; F8WEA9; H7BYU0
• Molecular Weight: 84,697 Da
• NCBI Official Full Name: Kinase suppressor of Ras 1
• NCBI Official Synonym Full Names: kinase suppressor of ras 1
• NCBI Official Symbol: KSR1
• NCBI Official Synonym Symbols: KSR; RSU2
• NCBI Protein Information: kinase suppressor of Ras 1
• UniProt Protein Name: Kinase suppressor of Ras 1
• Protein Family: Kinase suppressor of Ras
• UniProt Gene Name: KSR1
• UniProt Synonym Gene Names: KSR
• UniProt Entry Name: KSR1_HUMAN

Uniprot Description

KSR: a serine/threonine kinase of the Raf family with no demonstrated kinase activity. A location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. Interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. In unstimulated cells, the phosphorylated form is bound to a 14-3-3 protein and sequestered in the cytoplasm. Translocates from the cytoplasm to the cell periphery following TNF treatment. When Bad is available, ceramide signaling through the Ras/Raf cascade can lead to apoptosis. Three alternatively-spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; RAF family

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytosol; endoplasmic reticulum; membrane; protein complex

Molecular Function: MAP-kinase scaffold activity; protein binding; protein C-terminus binding

Biological Process: cAMP-mediated signaling; MAPKKK cascade; positive regulation of MAPKKK cascade; Ras protein signal transduction