Human keratin 72 ELISA Kit | KRT72 elisa kit

Human Keratin, type II cytoskeletal 72, KRT72 ELISA Kit

Cat. Num. AAA9341482

• Gene Names: KRT72; KRT6; K6irs; K6IRS2; KRT6IRS2
• Reactivity: Human
• Synonyms: keratin 72; Human Keratin; type II cytoskeletal 72; KRT72 ELISA Kit; type II cytoskeletal 72 (KRT72) ELISA kit; K6IRS2; K6irs; KRT6; KRT6IRS2; MGC142246; keratin 6; inner root sheath; 2; keratin protein K6irs; KRT72 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human KRT72 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml - 2000 pg/ml.
• Sensitivity: 10 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated KRT72 concentrations in Human serum, plasma and other body fluids. Using Purified Human KRT72 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add KRT72 and KRT72 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KRT72 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for KRT72 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated KRT72 concentrations in Human serum, plasma and other body fluids. Using Purified Human KRT72 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add KRT72 and KRT72 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KRT72 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 226246663
• NCBI GeneID: 140807
• NCBI Accession #: NP_001139697.1
• NCBI GenBank Nucleotide #: NM_001146225.1
• UniProt Accession #: Q14CN4; Q8NA87; Q8WWY9; Q8WWZ0; B4DEI8; H9KV51
• Molecular Weight: 55,877 Da
• NCBI Official Full Name: keratin, type II cytoskeletal 72 isoform 1
• NCBI Official Synonym Full Names: keratin 72
• NCBI Official Symbol: KRT72
• NCBI Official Synonym Symbols: KRT6; K6irs; K6IRS2; KRT6IRS2
• NCBI Protein Information: keratin, type II cytoskeletal 72; K72; CK-72; keratin-72; cytokeratin-72; type-II keratin Kb35; keratin protein K6irs; keratin 6, inner root sheath, 2; type II inner root sheath-specific keratin-K6irs2
• UniProt Protein Name: Keratin, type II cytoskeletal 72
• Protein Family: Keratin
• UniProt Gene Name: KRT72
• UniProt Synonym Gene Names: K6IRS2; KB35; KRT6; KRT6IRS2; CK-72; K72
• UniProt Entry Name: K2C72_HUMAN

NCBI Description

Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. The type II keratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This gene encodes a type II keratin that is specifically expressed in the inner root sheath of hair follicles. The type II keratins are clustered in a region of chromosome 12q12-q13. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2009]

Uniprot Description

KRT72: Has a role in hair formation. Specific component of keratin intermediate filaments in the inner root sheath (IRS) of the hair follicle (Probable). Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: keratin filament

Molecular Function: structural molecule activity