KLRB1 recombinant protein

Recombinant Human KLRB1 Protein

Cat. Num. AAA8249353

• Gene Names: KLRB1; NKR; CD161; CLEC5B; NKR-P1; NKRP1A; NKR-P1A; hNKR-P1A
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: KLRB1; Recombinant Human KLRB1 Protein; CLEC5B; NKRP1A; Killer cell lectin-like receptor subfamily B member 1; C-type lectin domain family 5 member B; HNKR-P1a; NKR-P1A; Natural killer cell surface protein P1A; CD161; KLRB1 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of PBS, pH 7.4.
• Sequence: QKSSIEKCSV DIQQSRNKTT ERPGLLNCPI YWQQLREKCL LFSHTVNPWN NSLADCSTKE SSLLLIRDKD ELIHTQNLIR DKAILFWIGL NFSLSEKNWK WINGSFLNSN DLEIRGDAKE NSCISISQTS VYSEYCSTEI RWICQKELTP VRNKVYPDSV DDIEGRMDEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK
• Sequence Length: 225

• Applicable Applications for KLRB1 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for KLRB1 recombinant protein

Recombinant Human KLRB1 Protein is produced by our mammalian expression system and the target gene encoding Gln67-Ser225 is expressed with a Fc tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4504879
• NCBI GeneID: 3820
• NCBI Accession #: NP_002249.1
• NCBI GenBank Nucleotide #: NM_002258.2
• UniProt Accession #: Q12918; Q24K24
• Molecular Weight: 25,415 Da
• NCBI Official Full Name: killer cell lectin-like receptor subfamily B member 1
• NCBI Official Synonym Full Names: killer cell lectin like receptor B1
• NCBI Official Symbol: KLRB1
• NCBI Official Synonym Symbols: NKR; CD161; CLEC5B; NKR-P1; NKRP1A; NKR-P1A; hNKR-P1A
• NCBI Protein Information: killer cell lectin-like receptor subfamily B member 1
• UniProt Protein Name: Killer cell lectin-like receptor subfamily B member 1
• Protein Family: Killer cell lectin-like receptor subfamily
• UniProt Gene Name: KLRB1
• UniProt Synonym Gene Names: CLEC5B; NKRP1A; NKR-P1A
• UniProt Entry Name: KLRB1_HUMAN

NCBI Description

Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. [provided by RefSeq, Jul 2008]

Uniprot Description

KLRB1: Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: plasma membrane

Molecular Function: transmembrane receptor activity

Biological Process: cell surface receptor linked signal transduction; regulation of immune response