Typical Testing Data/Standard Curve (for reference only)
Chicken Kallikrein 8 ELISA Kit | KLK8 elisa kit
Chicken Kallikrein 8 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This KLK8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken KLK8. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY KLK8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KLK8 antibody and an KLK8-HRP conjugate. The assay sample and buffer are incubated together with KLK8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLK8 concentration since KLK8 from samples and KLK8-HRP conjugate compete for the anti-KLK8 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLK8 from the sample, fewer sites are left to bind KLK8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLK8 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer. [provided by RefSeq, Jul 2008]
Uniprot Description
KLK8: Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer- collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. Belongs to the peptidase S1 family. Kallikrein subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; Cell development/differentiation; Secreted; EC 3.4.21.118; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: extracellular space; cytoplasm
Molecular Function: protein binding; serine-type endopeptidase activity
Biological Process: cell death; negative regulation of myelination; negative regulation of axon regeneration; response to wounding; synapse organization and biogenesis; regulation of synapse organization and biogenesis; keratinocyte proliferation; proteolysis; memory; neurite morphogenesis
Research Articles on KLK8
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Product Notes
The Chicken KLK8 klk8 (Catalog #AAA738663) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA738663 ELISA Kit recognizes Chicken KLK8. It is sometimes possible for the material contained within the vial of "Kallikrein 8, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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