Kruppel-Like Factor 4 Recombinant Protein | KLF4 recombinant protein

Recombinant Human Kruppel-Like Factor 4

Cat. Num. AAA144902

• Gene Names: KLF4; EZF; GKLF
• Purity: Greater than 80% as determined by SDS-PAGE.
• Synonyms: Kruppel-Like Factor 4; Recombinant Human Kruppel-Like Factor 4; KLF4 Human; Kruppel-Like Factor 4 (gut); EZF; GKLF; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor; endothelial kruppel-like zinc finger protein.; KLF4 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 80% as determined by SDS-PAGE.
• Form/Format: The KLF4 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MAHHHHHHMA DQLTEEQIAE FKEAFSLFDK DGDGTITTKE LGTVMRSLGQ NPTEAELQDM INEVDADGNG TIDFPEFLTM MARKMKDTDS EEEIREAFRV FDKDGNGYIS AAELRHVMTN LGEKLTDEEV DEMIREADID GDGQVNYEEF VQMMTAKGSM AVSDALLPSF STFASGPAGR EKTLRQAGAP NNRWREELSH MKRLPPVLPG RPYDLAAATV ATDLESGGAG AACGGSNLAP LPRRETEEFN DLLDLDFILS NSLTHPPESV AATVSSSASA SSSSSPSSSG PASAPSTCSF TYPIRAGNDP GVAPGGTGGG LLYGRESAPP PTAPFNLADI NDVSPSGGFV AELLRPELDP VYIPPQQPQP PGGGLMGKFV LKASLSAPGS EYGSPSVISV SKGSPDGSHP VVVAPYNGGP PRTCPKIKQE AVSSCTHLGA GPPLSNGHRP AAHDFPLGRQ LPSRTTPTLG LEEVLSSRDC HPALPLPPGF HPHPGPNYPS FLPDQMQPQV PPLHYQELMP PGSCMPEEPK PKRGRRSWPR KRTAT
• Sequence Length: 479

• Preparation and Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for KLF4 recombinant protein

Description: KLF4 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 545 amino acids (11-395) and having a molecular mass of 58.1 kDa. The KLF4 is fused to a 159 amino acid His-CaM Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Categories/Family for KLF4 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Kruppel-Like Factor

NCBI and Uniprot Product Information

• NCBI GI #: 194248077
• NCBI GeneID: 9314
• NCBI Accession #: NP_004226.3
• NCBI GenBank Nucleotide #: NM_004235.4
• UniProt Accession #: O43474; P78338; Q5T3J8; Q5T3J9; Q8N717; Q9UNP3; B2R8S4; B3KT79; L0R3I6; L0R4N5
• NCBI Official Full Name: Krueppel-like factor 4
• NCBI Official Synonym Full Names: Kruppel-like factor 4 (gut)
• NCBI Official Symbol: KLF4
• NCBI Official Synonym Symbols: EZF; GKLF
• NCBI Protein Information: Krueppel-like factor 4; endothelial Kruppel-like zinc finger protein; epithelial zinc finger protein EZF; gut-enriched krueppel-like factor
• UniProt Protein Name: Krueppel-like factor 4
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF4
• UniProt Synonym Gene Names: EZF; GKLF
• UniProt Entry Name: KLF4_HUMAN

Uniprot Description

KLF4: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain). Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin

Molecular Function: protein binding; zinc ion binding; double-stranded DNA binding; phosphoinositide 3-kinase regulator activity; transcription factor activity

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; post-embryonic camera-type eye development; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of muscle hyperplasia; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of hemoglobin biosynthetic process; regulation of phosphoinositide 3-kinase activity; positive regulation of protein metabolic process; negative regulation of cell proliferation; post-embryonic hemopoiesis; epidermis morphogenesis; regulation of cell differentiation; negative regulation of protein kinase B signaling cascade; response to retinoic acid; negative regulation of heterotypic cell-cell adhesion; stem cell maintenance; positive regulation of telomerase activity; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; mesodermal cell fate determination; negative regulation of phosphoinositide 3-kinase cascade