Mouse SCF ELISA Kit | KITLG elisa kit

Mouse SCF PicoKine ELISA Kit

Cat. Num. AAA177279

• Gene Names: Kitl; Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted
• Reactivity: Mouse
• Synonyms: SCF; Mouse SCF PicoKine ELISA Kit; Kit ligand; KIT ligand; C kit ligand; Ckit ligand; Kitl; KITLG; KL 1; KL1; Mast cell growth factor; MGF; SF; SHEP7; Steel factor; Stem cell factor; Stem cell factor precursor; KITLG elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse SCF
• Sequence Length: 273

• Samples: Cell culture supernates, serum and plasma (EDTA)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse SCF PicoKine ELISA Kit standard curve )

Related Product Information for KITLG elisa kit

Principle of the assay: mouse SCF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SCF has been precoated onto 96-well plates. Standards(E.coli, K26-A190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SCF amount of sample captured in plate.
Background: Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.

NCBI and Uniprot Product Information

• NCBI GI #: 134290
• NCBI GeneID: 17311
• UniProt Accession #: P20826; P97332; Q3TNJ7; Q62524; Q64222; Q921N5
• Molecular Weight: 27,614 Da
• NCBI Official Full Name: Kit ligand
• NCBI Official Synonym Full Names: kit ligand
• NCBI Official Symbol: Kitl
• NCBI Official Synonym Symbols: Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted
• NCBI Protein Information: kit ligand; cloud gray; C-kit ligand; Steel factor; grizzle-belly; stem cell factor; mast cell growth factor; hematopoietic growth factor KL
• UniProt Protein Name: Kit ligand
• Protein Family: SCF-associated factor
• UniProt Gene Name: Kitlg
• UniProt Synonym Gene Names: Kitl; Mgf; Sl; Slf; MGF; SCF; sKITLG
• UniProt Entry Name: SCF_MOUSE

Uniprot Description

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; plasma membrane; extracellular region; integral to membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: embryonic hemopoiesis; positive regulation of leukocyte migration; germ cell programmed cell death; germ cell development; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; ovarian follicle development; positive regulation of melanocyte differentiation; negative regulation of mast cell apoptosis; positive regulation of cell proliferation; positive regulation of myeloid leukocyte differentiation; positive regulation of Ras protein signal transduction; cell adhesion; neural crest cell migration; positive regulation of DNA replication; negative regulation of apoptosis