KIN17 recombinant protein

KIN17, 1-393aa, Human, His tag, E Coli

Cat. Num. AAA205498

• Gene Names: KIN; BTCD; Rts2; KIN17
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: KIN17; 1-393aa; Human; His tag; E Coli; DNA/RNA-binding protein KIN17; BTCD; KIN; KIN17 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGKSDFL TPKAIANRIK SKGLQKLRWY CQMCQKQCRD ENGFKCHCMS ESHQRQLLLA SENPQQFMDY FSEEFRNDFL ELLRRRFGTK RVHNNIVYNE YISHREHIHM NATQWETLTD FTKWLGREGL CKVDETPKGW YIQYIDRDPE TIRRQLELEK KKKQDLDDEE KTAKFIEEQV RRGLEGKEQE VPTFTELSRE NDEEKVTFNL SKGACSSSGA TSSKSSTLGP SALKTIGSSA SVKRKESSQS STQSKEKKKK KSALDEIMEI EEEKKRTART DYWLQPEIIV KIITKKLGEK YHKKKAIVKE VIDKYTAVVK MIDSGDKLKL DQTHLETVIP APGKRILVLN GGYRGNEGTL ESINEKTFSA TIVIETGPLK GRRVEGIQYE DISKLA
• Sequence Length: 393

• Applicable Applications for KIN17 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for KIN17 recombinant protein

DNA/RNA-binding protein KIN17, also known as KIN17, is a nuclear protein that forms intranuclear foci during proliferation and is redistributed in the nucleoplasm during the cell cycle. KIN17 is ubiqutiously expressed with the highest levels of expression in muscle, heart and testis. SV40-transformed fibroblasts overexpress KIN17, which interacts with Large T antigen and reduces T-antigen-dependent DNA replication. Recombinant human KIN17 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for KIN17 recombinant protein

Epigenomics

References

Biard D S., et al. (1997) Radiat Res. 147:42-450; Mazin A., et al. (1994) Nucleic Acids Res. 22: 4335-4341.;

NCBI and Uniprot Product Information

• NCBI GI #: 13124883
• NCBI GeneID: 22944
• NCBI Accession #: NP_036443
• NCBI GenBank Nucleotide #: NM_012311.3
• UniProt Accession #: O60870; B4DX32
• Molecular Weight: 47.8 kDa (416aa) confirmed by MALDI-TOF
• NCBI Official Full Name: DNA/RNA-binding protein KIN17
• NCBI Official Synonym Full Names: Kin17 DNA and RNA binding protein
• NCBI Official Symbol: KIN
• NCBI Official Synonym Symbols: BTCD; Rts2; KIN17
• NCBI Protein Information: DNA/RNA-binding protein KIN17
• UniProt Protein Name: DNA/RNA-binding protein KIN17
• Protein Family: KIN17-like protein
• UniProt Gene Name: KIN

NCBI Description

The protein encoded by this gene is a nuclear protein that forms intranuclear foci during proliferation and is redistributed in the nucleoplasm during the cell cycle. Short-wave ultraviolet light provokes the relocalization of the protein, suggesting its participation in the cellular response to DNA damage. Originally selected based on protein-binding with RecA antibodies, the mouse protein presents a limited similarity with a functional domain of the bacterial RecA protein, a characteristic shared by this human ortholog. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (). Binds via its C-terminal domain to RNA in vitro.