SDS-Page
KDSR recombinant protein
KDSR, 26-270aa, Human, His tag, E Coli
Gene Names
KDSR; DHSR; FVT1; EKVP4; SDR35C1
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
KDSR; 26-270aa; Human; His tag; E Coli; 3-ketodihydrosphingosine reductase; DHSR; FVT1; KDSR recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl, 0.1mM PMSF
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MKPLALPGAH VVVTGGSSGI GKCIAIECYK QGAFITLVAR NEDKLLQAKK EIEMHSINDK QVVLCISVDV SQDYNQVENV IKQAQEKLGP VDMLVNCAGM AVSGKFEDLE VSTFERLMSI NYLGSVYPSR AVITTMKERR VGRIVFVSSQ AGQLGLFGFT AYSASKFAIR GLAEALQMEV KPYNVYITVA YPPDTDTPGF AEENRTKPLE TRLISETTSV CKPEQVAKQI VKDAIQGNFN SSLGSD
Sequence Length
332
Applicable Applications for KDSR recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for KDSR recombinant protein
KDSR, also known as 3-ketodihydrosphingosine reductase, is a 332 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum (ER) and belongs to the short-chain dehydrogenases/reductases (SDR) family. KDSR is a secreted protein which is weakly expressed in hematopoietic tissue. Also this protein catalyzes the reduction of 3-ketodihydrosphingosine (KDS) to dihydrosphingosine (DHS) Recombinant human KDSR protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for KDSR recombinant protein
References
Kihara A., et al. (2004) J. Biol. Chem. 279:49243-49250; Parkinson, N.J., et al. (2008) Neuromuscul. Disord. 18: 394-397.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29.0 kDa (266aa) confirmed by MALDI-TOF
NCBI Official Full Name
3-ketodihydrosphingosine reductase
NCBI Official Synonym Full Names
3-ketodihydrosphingosine reductase
NCBI Official Symbol
KDSR
NCBI Official Synonym Symbols
DHSR; FVT1; EKVP4; SDR35C1
NCBI Protein Information
3-ketodihydrosphingosine reductase
UniProt Protein Name
3-ketodihydrosphingosine reductase
UniProt Gene Name
KDSR
UniProt Synonym Gene Names
FVT1; SDR35C1; KDS reductase; FVT-1
NCBI Description
The protein encoded by this gene catalyzes the reduction of 3-ketodihydrosphingosine to dihydrosphingosine. The putative active site residues of the encoded protein are found on the cytosolic side of the endoplasmic reticulum membrane. A chromosomal rearrangement involving this gene is a cause of follicular lymphoma, also known as type II chronic lymphatic leukemia. The mutation of a conserved residue in the bovine ortholog causes spinal muscular atrophy. [provided by RefSeq, Jul 2008]
Uniprot Description
Catalyzes the reduction of 3-ketodihydrosphingosine (KDS) to dihydrosphingosine (DHS).
Research Articles on KDSR
Similar Products
Product Notes
The KDSR kdsr (Catalog #AAA203775) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's KDSR can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the KDSR kdsr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MKPLALPGAH VVVTGGSSGI GKCIAIECYK QGAFITLVAR NEDKLLQAKK EIEMHSINDK QVVLCISVDV SQDYNQVENV IKQAQEKLGP VDMLVNCAGM AVSGKFEDLE VSTFERLMSI NYLGSVYPSR AVITTMKERR VGRIVFVSSQ AGQLGLFGFT AYSASKFAIR GLAEALQMEV KPYNVYITVA YPPDTDTPGF AEENRTKPLE TRLISETTSV CKPEQVAKQI VKDAIQGNFN SSLGSD. It is sometimes possible for the material contained within the vial of "KDSR, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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