Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) Recombinant Protein | VEGFR2 recombinant protein

Recombinant Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)

Cat. Num. AAA2009067

• Gene Names: Kdr; Flk1; Ly73; Flk-1; Krd-1; VEGFR2; VEGFR-2; sVEGFR-2; 6130401C07
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Vascular Endothelial Growth Factor Receptor 2 (VEGFR2); Recombinant Vascular Endothelial Growth Factor Receptor 2 (VEGFR2); VEGFR2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-NTTLQITCRG QRDLDWLWPN AQRDSEERVL VTECGGGDSI FCKTLTIPRV VGNDTGAYKC SYRDVDIAST VYVYVRDYRS PFIASVSDQH GIVYITENKN KTVVIPCRGS ISNLNVSLCA RYPEKRFVPD GNRISWDSEI GFTLPSYMIS YAGMVFCEAK INDETYQSIM YIVVVVGYRI YDVILSPPHE IELSAGEKLV LNCTARTELN VGLDFTWHSP PSKSHHKKIV NRDVKPFPGT VAKMFLSTLT IESVTKSDQG EYTCVASSGR MIKRNRT
• Sequence Length: 1367

• Applicable Applications for VEGFR2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Asn46~Thr322 (Accession # P35918) with N-terminal His-Tag
• Predicted isoelectric point: 7.6
• Accurate Molecular Mass: 35kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 549321
• NCBI GeneID: 16542
• UniProt Accession #: P35918; C5H7S5
• Molecular Weight: 32.7kDa
• NCBI Official Full Name: Vascular endothelial growth factor receptor 2
• NCBI Official Synonym Full Names: kinase insert domain protein receptor
• NCBI Official Symbol: Kdr
• NCBI Official Synonym Symbols: Flk1; Ly73; Flk-1; Krd-1; VEGFR2; VEGFR-2; sVEGFR-2; 6130401C07
• NCBI Protein Information: vascular endothelial growth factor receptor 2; kinase NYK; VEGF receptor-2; fetal liver kinase 1; protein-tyrosine kinase receptor flk-1; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; vascular endothelial growth factor receptor- 2; soluble vascular endothelial growth factor receptor 2
• UniProt Protein Name: Vascular endothelial growth factor receptor 2
• UniProt Gene Name: Kdr
• UniProt Synonym Gene Names: Flk-1; Flk1; VEGFR-2; FLK-1
• UniProt Entry Name: VGFR2_MOUSE

Uniprot Description

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; VEGFR family

Cellular Component: Golgi apparatus; neuron projection; cell surface; integral to plasma membrane; endoplasmic reticulum; early endosome; extracellular region; integral to membrane; cytosol; lipid raft; cell soma; membrane; cytoplasm; plasma membrane; cytoplasmic vesicle; cell junction; nucleus; endosome; external side of plasma membrane

Molecular Function: transferase activity; integrin binding; vascular endothelial growth factor receptor activity; protein binding; protein-tyrosine kinase activity; growth factor binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of positive chemotaxis; multicellular organismal development; cell maturation; positive regulation of vasodilation; protein amino acid phosphorylation; positive regulation of long-term neuronal synaptic plasticity; calcium ion homeostasis; regulation of cell shape; elevation of cytosolic calcium ion concentration; positive regulation of mesenchymal cell proliferation; negative regulation of neuron apoptosis; positive regulation of TOR signaling pathway; response to drug; regulation of endothelial cell proliferation; cell fate commitment; embryonic hemopoiesis; regulation of endothelial cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; branching morphogenesis of a tube; positive regulation of endothelial cell proliferation; lymph vessel development; alveolus development; surfactant homeostasis; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of nitric-oxide synthase biosynthetic process; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of calcium-mediated signaling; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of MAPKKK cascade; positive regulation of focal adhesion formation; ovarian follicle development; positive regulation of cell proliferation; hemopoiesis; angiogenesis; vasculogenesis; cell differentiation; positive regulation of BMP signaling pathway; endothelial cell differentiation; cell migration; male gonad development; response to hypoxia; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; neurite morphogenesis; lung development; positive regulation of cell migration