Human Potassium channel subfamily K member 3 ELISA Kit | KCNK3 elisa kit

Human Potassium channel subfamily K member 3 (KCNK3) ELISA Kit

Cat. Num. AAA281462

• Gene Names: KCNK3; OAT1; PPH4; TASK; TBAK1; K2p3.1; TASK-1
• Reactivity: Human
• Synonyms: Potassium channel subfamily K member 3; Human Potassium channel subfamily K member 3 (KCNK3) ELISA Kit; KCNK3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human KCNK3. No significant cross-reactivity or interference between Human KCNK3 and analogues was observed.
• Sequence Length: 394

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for KCNK3 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KCNK3 in samples. An antibody specific for KCNK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKCNK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCNK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4504849
• NCBI GeneID: 3777
• NCBI Accession #: NP_002237.1
• NCBI GenBank Nucleotide #: NM_002246.2
• UniProt Accession #: O14649; Q53SU2
• Molecular Weight: 43,518 Da
• NCBI Official Full Name: potassium channel subfamily K member 3
• NCBI Official Synonym Full Names: potassium two pore domain channel subfamily K member 3
• NCBI Official Symbol: KCNK3
• NCBI Official Synonym Symbols: OAT1; PPH4; TASK; TBAK1; K2p3.1; TASK-1
• NCBI Protein Information: potassium channel subfamily K member 3
• UniProt Protein Name: Potassium channel subfamily K member 3
• Protein Family: Potassium channel subfamily
• UniProt Gene Name: KCNK3
• UniProt Synonym Gene Names: TASK; TASK1; Two pore K(+) channel KT3.1

NCBI Description

This gene encodes a member of the superfamily of potassium channel proteins that contain two pore-forming P domains. The encoded protein is an outwardly rectifying channel that is sensitive to changes in extracellular pH and is inhibited by extracellular acidification. Also referred to as an acid-sensitive potassium channel, it is activated by the anesthetics halothane and isoflurane. Although three transcripts are detected in northern blots, there is currently no sequence available to confirm transcript variants for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

KCNK3: pH-dependent, voltage-insensitive, background potassium channel protein. Rectification direction results from potassium ion concentration on either side of the membrane. Acts as an outward rectifier when external potassium concentration is low. When external potassium concentration is high, current is inward. Widespread expression in adult. Strongest expression in pancreas and placenta. Lower expression in brain, lung, prostate, heart, kidney, uterus, small intestine and colon. Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.

Protein type: Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: extracellular space; integral component of plasma membrane; plasma membrane

Molecular Function: growth factor activity; ion channel activity; open rectifier potassium channel activity; potassium channel activity; potassium ion leak channel activity; protein binding; protein C-terminus binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: brain development; potassium ion transport; reduction of cytosolic calcium ion concentration; response to drug; stabilization of membrane potential; synaptic transmission

Disease: Pulmonary Hypertension, Primary, 4