Brevetoxin B Reagent | LEPREL2 reagent

Brevetoxin B (pbTx-2)

Cat. Num. AAA635353

• Gene Names: LEPREL2; GRCB; P3H3; HSU47926
• Applications: ELISA
• Purity: Highly Purified; ~95% by HPLC
• Synonyms: Brevetoxin B; Brevetoxin B (pbTx-2); LEPREL2 reagent

• Host: Karenia brevis
• Purity/Purification: Highly Purified; ~95% by HPLC
• Form/Format: Supplied as a white solid.

• Applicable Applications for LEPREL2 reagent: ELISA (EL/EIA)
• CAS Number: 79580-28-2
• Molecular Formula: C50H70O14
• Solubility: Acetone, ethyl acetate, 100% methanol, 100% ethanol. Can be subsequently diluted into aqueous solutions for biological studies.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile buffer. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.; This product does not contain any animal derived additives, such as albumin.

Structure

(Structure of B2739-85|Brevetoxin B (pbTx-2))

Related Product Information for LEPREL2 reagent

A polycyclic polyether, isolated from marine dinoflagellate Karenia breve (Ptychodiscus brevis, or Gymnodinium breve in earlier literature) is one of the toxins responsible for the 'red tide' massive fish kills. This compound forms a ladder-like-arranged multiple ring structure with an unusual acrolein side-chain on one of the terminal rings. Together with other congeners, they are known to disrupt neurotransmission by opening sodium channels.

Brevetoxin B is a neurotoxin produced by algae called Ptychodiscus brevis Davis or Gymnodinium breve Davis. The algae proliferate during red tide incidents, vast phytoplankton blooms that actually turn the sea red, brown, or green. For example, 14 humpback whales died and many people were sickened by a red tide incident in 1987 in Cape Cod Bay, Mass. Brevetoxin B exerts its neurotoxic effects by binding to sodium channels on nerve and muscle cell membranes, causing an excessive influx of sodium ions across the membranes, which leads to cell death.

Product Categories/Family for LEPREL2 reagent

Molecular Biology; MB-Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 1200503
• NCBI GeneID: 10536
• UniProt Accession #: Q8IVL6; Q13512; Q15740; Q66K32; Q6NX61; Q7L2T1
• Molecular Weight: 895.1
• NCBI Official Full Name: B
• NCBI Official Synonym Full Names: leprecan-like 2
• NCBI Official Symbol: LEPREL2
• NCBI Official Synonym Symbols: GRCB; P3H3; HSU47926
• NCBI Protein Information: prolyl 3-hydroxylase 3; protein B; gene rich cluster, B
• UniProt Protein Name: Prolyl 3-hydroxylase 3
• UniProt Gene Name: LEPREL2
• UniProt Synonym Gene Names: P3H3
• UniProt Entry Name: P3H3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the leprecan family of proteoglycans, which function as collagen prolyl hydroxylases that are required for proper collagen biosynthesis, folding and assembly. This protein, like other family members, is thought to reside in the endoplasmic reticulum. Epigenetic inactivation of this gene is associated with breast and other cancers, suggesting that it may function as a tumor suppressor. [provided by RefSeq, Aug 2013]

Uniprot Description

LEPREL2: Has prolyl 3-hydroxylase activity catalyzing the post- translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. Belongs to the leprecan family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.7; Oxidoreductase

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: endoplasmic reticulum lumen

Molecular Function: procollagen-proline 3-dioxygenase activity; L-ascorbic acid binding; iron ion binding

Biological Process: negative regulation of cell proliferation; extracellular matrix organization and biogenesis; peptidyl-proline hydroxylation