Human Izumo sperm-egg fusion protein 1 ELISA Kit | IZUMO1 elisa kit

Human Izumo sperm-egg fusion protein 1 ELISA Kit

Cat. Num. AAA761720

• Gene Names: IZUMO1; IZUMO
• Reactivity: Human
• Synonyms: Izumo sperm-egg fusion protein 1; Human Izumo sperm-egg fusion protein 1 ELISA Kit; IZUMO1 (Izumo sperm-egg fusion protein 1)/OBF/Oocyte binding/fusion factor/Sperm-specific protein izumo; IZUMO1 elisa kit

• Reactivity: Human
• Sequence Length: 350

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for IZUMO1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IZUMO1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IZUMO1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IZUMO1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IZUMO1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 194097475
• NCBI GeneID: 284359
• NCBI Accession #: NP_872381.2
• NCBI GenBank Nucleotide #: NM_182575.2
• Molecular Weight: 7,339 Da
• NCBI Official Full Name: izumo sperm-egg fusion protein 1
• NCBI Official Synonym Full Names: izumo sperm-egg fusion 1
• NCBI Official Symbol: IZUMO1
• NCBI Official Synonym Symbols: IZUMO
• NCBI Protein Information: izumo sperm-egg fusion protein 1
• UniProt Protein Name: Izumo sperm-egg fusion protein 1
• Protein Family: Izumo sperm-egg fusion protein
• UniProt Gene Name: IZUMO1
• UniProt Synonym Gene Names: OBF
• UniProt Entry Name: IZUM1_HUMAN

NCBI Description

The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasma membrane binding and fusion (Inoue et al., 2005 [PubMed 15759005]).[supplied by OMIM, Mar 2008]

Uniprot Description

IZUMO1: Involved in the fertilization process. May be involved in the fusion of the sperm with the egg. Belongs to the Izumo family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: acrosomal membrane; integral to membrane; plasma membrane

Molecular Function: protein homodimerization activity; receptor binding

Biological Process: cell adhesion; fusion of sperm to egg plasma membrane; multicellular organism reproduction; single fertilization; sperm-egg recognition