Integrin beta1 ELISA Kit | ITGB1 elisa kit

Integrin beta1 Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500319

• Gene Names: ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• Reactivity: Human, Mouse, Rat
• Synonyms: Integrin beta1; Integrin beta1 Colorimetric Cell-Based ELISA Kit; CD29; FNRB; Fibronectin receptor beta subunit; ITB1; Integrin VLA-4 beta subunit; Integrin beta-1 precursor; integrin beta-1; ITGB1 elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: One 96-Well Plate
• Sequence Length: 798

• Preparation and Storage: Store at 4 degree C.

Related Product Information for ITGB1 elisa kit

Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Product Categories/Family for ITGB1 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 19743813
• NCBI GeneID: 3688
• NCBI Accession #: NP_002202.2
• NCBI GenBank Nucleotide #: NM_002211.3
• UniProt Accession #: P05556; P78466; P78467; Q13089; Q13090; Q13091; Q13212; A8K6N2; D3DRX9; D3DRY3; D3DRY4; D3DRY5
• Molecular Weight: 88465
• NCBI Official Full Name: integrin beta-1 isoform 1A
• NCBI Official Synonym Full Names: integrin subunit beta 1
• NCBI Official Symbol: ITGB1
• NCBI Official Synonym Symbols: CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• NCBI Protein Information: integrin beta-1
• UniProt Protein Name: Integrin beta-1
• Protein Family: Integrin
• UniProt Gene Name: ITGB1
• UniProt Synonym Gene Names: FNRB; MDF2; MSK12; GPIIA

NCBI Description

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Cell adhesion; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 10p11.22

Cellular Component: cell surface; cleavage furrow; cytoplasm; dendritic spine; external side of plasma membrane; extracellular exosome; filopodium; focal adhesion; integrin alpha1-beta1 complex; integrin alpha10-beta1 complex; integrin alpha11-beta1 complex; integrin alpha2-beta1 complex; integrin alpha3-beta1 complex; integrin alpha7-beta1 complex; integrin alpha8-beta1 complex; integrin complex; intercalated disc; invadopodium membrane; lamellipodium; melanosome; membrane; membrane raft; neuromuscular junction; perinuclear region of cytoplasm; plasma membrane; receptor complex; recycling endosome; ruffle; sarcolemma

Molecular Function: actin binding; C-X3-C chemokine binding; cadherin binding; cell adhesion molecule binding; collagen binding involved in cell-matrix adhesion; coreceptor activity; fibronectin binding; integrin binding; laminin binding; metal ion binding; protease binding; protein binding; protein complex binding; protein heterodimerization activity; viral receptor activity

Biological Process: axon extension; B cell differentiation; basement membrane organization; calcium-independent cell-matrix adhesion; cardiac muscle cell differentiation; cell adhesion mediated by integrin; cell fate specification; cell migration; cell migration during sprouting angiogenesis; cell-cell adhesion mediated by integrin; cell-matrix adhesion; cell-substrate adhesion; cellular defense response; cellular response to low-density lipoprotein particle stimulus; dendrite morphogenesis; entry of virus into host cell; extracellular matrix organization; formation of radial glial scaffolds; G1/S transition of mitotic cell cycle; germ cell migration; heterotypic cell-cell adhesion; homophilic cell adhesion; in utero embryonic development; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; mesodermal cell differentiation; negative regulation of anoikis; negative regulation of cell differentiation; negative regulation of Rho protein signal transduction; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of GTPase activity; positive regulation of protein kinase B signaling; positive regulation of receptor activity; receptor internalization; regulation of cell cycle; regulation of collagen catabolic process; regulation of immune response; sarcomere organization; stress fiber assembly; transforming growth factor beta receptor signaling pathway; visual learning