Integrin alpha 6 beta 4 Recombinant Protein | ITGA6&ITGB4 recombinant protein

Integrin alpha 6 beta 4 Protein, Human, Recombinant (DYKDDDDK & His Tag)

Cat. Num. AAA8123623

• Purity: >80% as determined by SDS-PAGE
• Synonyms: Integrin alpha 6 beta 4; Integrin alpha 6 beta 4 Protein; Human; Recombinant (DYKDDDDK & His Tag); Human ITGA6 & ITGB4 Heterodimer Protein; ITGA6&ITGB4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser1011&Met1-Ser710

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Phe 24 & Asn 28
• Tag: C-DYKDDDDK & C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Ser 1011) of human ITGA6 isoform alpha-6X1B (P23229-3) was fused with a DYKDDDDK tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Ser 710) of human ITGB4 (CAA37656.1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• UniProt Accession #: P23229
• Molecular Weight: The recombinant heterodimer of human ITGA6&ITGB4 comprises 1754 (1030 + 724) amino acids and has a calculated molecular mass of 196 (115 + 81)kDa.
• UniProt Protein Name: Integrin alpha-6
• UniProt Gene Name: ITGA6
• UniProt Entry Name: ITA6_HUMAN

Uniprot Description

ITGA6: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21. Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Belongs to the integrin alpha chain family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: cell surface; cell-cell adherens junction; focal adhesion; hemidesmosome; plasma membrane; basal plasma membrane; basement membrane; filopodium; external side of plasma membrane

Molecular Function: integrin binding; protein binding; metal ion binding; laminin binding

Biological Process: integrin-mediated signaling pathway; skin development; extracellular matrix organization and biogenesis; filopodium formation; positive regulation of apoptosis; cell-matrix adhesion; cell-substrate adhesion; cell-cell adhesion; hemidesmosome assembly; gut development; cellular response to extracellular stimulus; brown fat cell differentiation; positive regulation of cell-cell adhesion; cell-substrate junction assembly; cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; leukocyte migration; positive regulation of phosphorylation

Disease: Epidermolysis Bullosa Junctionalis With Pyloric Atresia