ITGA5 recombinant protein

Recombinant Human ITGA5 Protein

Cat. Num. AAA8249323

• Gene Names: ITGA5; FNRA; CD49e; VLA-5; VLA5A
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Synonyms: ITGA5; Recombinant Human ITGA5 Protein; FNRA; Integrin alpha-5; CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD49e; ITGA5 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence: FNLDAEAPAV LSGPPGSFFG FSVEFYRPGT DGVSVLVGAP KANTSQPGVL QGGAVYLCPW GASPTQCTPI EFDSKGSRLL ESSLSSSEGE EPVEYKSLQW FGATVRAHGS SILACAPLYS WRTEKEPLSD PVGTCYLSTD NFTRILEYAP CRSDFSWAAG QGYCQGGFSA EFTKTGRVVL GGPGSYFWQG QILSATQEQI AESYYPEYLI NLVQGQLQTR QASSIYDDSY LGYSVAVGEF SGDDTEDFVA GVPKGNLTYG YVTILNGSDI RSLYNFSGEQ MASYFGYAVA ATDVNGDGLD DLLVGAPLLM DRTPDGRPQE VGRVYVYLQH PAGIEPTPTL TLTGHDEFGR FGSSLTPLGD LDQDGYNDVA IGAPFGGETQ QGVVFVFPGG PGGLGSKPSQ VLQPLWAASH TPDFFGSALR GGRDLDGNGY PDLIVGSFGV DKAVVYRGRP IVSASASLTI FPAMFNPEER SCSLEGNPVA CINLSFCLNA SGKHVADSIG FTVELQLDWQ KQKGGVRRAL FLASRQATLT QTLLIQNGAR EDCREMKIYL RNESEFRDKL SPIHIALNFS LDPQAPVDSH GLRPALHYQS KSRIEDKAQI LLDCGEDNIC VPDLQLEVFG EQNHVYLGDK NALNLTFHAQ NVGEGGAYEA ELRVTAPPEA EYSGLVRHPG NFSSLSCDYF AVNQSRLLVC DLGNPMKAGA SLWGGLRFTV PHLRDTKKTI QFDFQILSKN LNNSQSDVVS FRLSVEAQAQ VTLNGVSKPE AVLFPVSDWH PRDQPQKEED LGPAVHHVYE LINQGPSSIS QGVLELSCPQ ALEGQQLLYV TRVTGLNCTT NHPINPKGLE LDPEGSLHHQ QKREAPSRSS ASSGPQILKC PEAECFRLRC ELGPLHQQES QSLQLHFRVW AKTFLQREHQ PFSLQCEAVY KALKMPYRIL PRQLPQKERQ VATAVQWTKA EGSYVDHHHH HH
• Sequence Length: 1049

• Applicable Applications for ITGA5 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for ITGA5 recombinant protein

Recombinant Human ITGA5 Protein is produced by our Mammalian expression system and the target gene encoding Phe42-Tyr995 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 1017029567
• NCBI GeneID: 3678
• NCBI Accession #: NP_002196.4
• NCBI GenBank Nucleotide #: NM_002205.4
• UniProt Accession #: P08648; Q96HA5
• Molecular Weight: 114,536 Da
• NCBI Official Full Name: integrin alpha-5
• NCBI Official Synonym Full Names: integrin subunit alpha 5
• NCBI Official Symbol: ITGA5
• NCBI Official Synonym Symbols: FNRA; CD49e; VLA-5; VLA5A
• NCBI Protein Information: integrin alpha-5
• UniProt Protein Name: Integrin alpha-5
• Protein Family: Integrin
• UniProt Gene Name: ITGA5
• UniProt Synonym Gene Names: FNRA
• UniProt Entry Name: ITA5_HUMAN

NCBI Description

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015]

Uniprot Description

ITGA5: Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with HIV- 1 Tat. Belongs to the integrin alpha chain family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q11-q13

Cellular Component: cell surface; focal adhesion; intercellular junction; plasma membrane; ruffle

Molecular Function: platelet-derived growth factor receptor binding; protein binding; vascular endothelial growth factor receptor 2 binding

Biological Process: angiogenesis; cell adhesion; cell adhesion mediated by integrin; cell-substrate adhesion; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; leukocyte migration; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; wound healing, spreading of epidermal cells