Integrin Alpha 10 (ITGa10) Recombinant Protein | ITGa10 recombinant protein

Recombinant Integrin Alpha 10 (ITGa10)

Cat. Num. AAA2009320

• Gene Names: ITGA10; PRO827
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Integrin Alpha 10 (ITGa10); Recombinant Integrin Alpha 10 (ITGa10); ITGa10 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG S-SSLLSQVFVK LTASSDSLER NGTLQDNTAQ TSAYIQYEPH LLFSSESTLH RYEVHPYGTL PVGPGPEFKT TLRVQNLGCY VVSGLIISAL LPAVAHGGNY FLSLSQVITN NASCIVQNLT EPPGPPVHPE ELQHTNRLNG SNTQCQVVRC HLGQLAKGTE VSVGLLRLVH NEFFRRAKFK SLTVVSTFEL GTEEGSVLQL TEASRWSE
• Sequence Length: 1167

• Applicable Applications for ITGa10 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ser901~Glu1108 (Accession # O75578) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 38569398
• NCBI GeneID: 8515
• NCBI Accession #: NP_003628.2
• NCBI GenBank Nucleotide #: NM_003637.3
• UniProt Accession #: O75578; Q6UXJ6; Q9UHZ8; B2RAM4
• Molecular Weight: 24.0kDa
• NCBI Official Full Name: integrin alpha-10
• NCBI Official Synonym Full Names: integrin, alpha 10
• NCBI Official Symbol: ITGA10
• NCBI Official Synonym Symbols: PRO827
• NCBI Protein Information: integrin alpha-10
• UniProt Protein Name: Integrin alpha-10
• Protein Family: Integrin
• UniProt Gene Name: ITGA10
• UniProt Entry Name: ITA10_HUMAN

NCBI Description

Integrins are integral transmembrane glycoproteins composed of noncovalently linked alpha and beta chains. They participate in cell adhesion as well as cell-surface mediated signalling. This gene encodes an integrin alpha chain and is expressed at high levels in chondrocytes, where it is transcriptionally regulated by AP-2epsilon and Ets-1. The protein encoded by this gene binds to collagen. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

ITGA10: Integrin alpha-10/beta-1 is a receptor for collagen. Belongs to the integrin alpha chain family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: plasma membrane; integrin complex

Molecular Function: collagen binding; metal ion binding

Biological Process: integrin-mediated signaling pathway; axon guidance; extracellular matrix organization and biogenesis; cell-matrix adhesion; blood coagulation