IRF2 recombinant protein

IRF2, human recombinant

Cat. Num. AAA844775

• Gene Names: IRF2; IRF-2
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: IRF2; human recombinant; Interferon regulatory factor-2; IRF2 recombinant protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT and 10% glycerol.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MPVERMRMRP WLEEQINSNT IPGLKWLNKE KKIFQIPWMH AARHGWDVEK DAPLFRNWAI HTGKHQPGVD KPDPKTWKAN FRCAMNSLPD IEEVKDKSIK KGNNAFRVYR MLP
• Sequence Length: 349

• Applicable Applications for IRF2 recombinant protein: SDS-PAGE
• Endotoxin Level: < 1.0 EU per 1 microgram of protein
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for IRF2 recombinant protein

Background: Interferon regulatory factor (IRF) 2 is a member of the interferon regulatory transcription factor family. IRF-2 is generally regarded as an oncoprotein. Structure of IRF1 and IRF2 are similar. However, its functions are different. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. Recombinant human IRF2 was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for IRF2 recombinant protein

Cytokines; Growth Factors & Hormones; IFNsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; IFNsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth

NCBI and Uniprot Product Information

• NCBI GI #: 153082752
• NCBI GeneID: 3660
• NCBI Accession #: NP_002190.2
• NCBI GenBank Nucleotide #: NM_002199.3
• UniProt Accession #: P14316; Q6IAS7; Q96B99; D6RCK5; H0Y8S3
• Molecular Weight: 15 kDa (133 aa, 1-113 aa + His tag), confirmed by MALDI-TOF.
• NCBI Official Full Name: interferon regulatory factor 2
• NCBI Official Synonym Full Names: interferon regulatory factor 2
• NCBI Official Symbol: IRF2
• NCBI Official Synonym Symbols: IRF-2
• NCBI Protein Information: interferon regulatory factor 2
• UniProt Protein Name: Interferon regulatory factor 2
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF2
• UniProt Synonym Gene Names: IRF-2
• UniProt Entry Name: IRF2_HUMAN

NCBI Description

IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4. [provided by RefSeq, Jul 2008]

Uniprot Description

IRF2: Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation. Interacts with BRD7, IRF2BP1 and IRF2BP2. Interacts with CREBBP in growing cells; the interaction acetylates IRF2 and regulates IRF2-dependent H4 promoter activity. By viruses and IFN. Expressed throughout the epithelium of the colon. Also expressed in lamina propria. Belongs to the IRF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 4q34.1-q35.1

Cellular Component: nucleoplasm; focal adhesion; cytoplasm; cytosol

Molecular Function: protein binding; DNA binding; transcription factor activity

Biological Process: cell proliferation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; blood coagulation