Porcine Insulin Like Protein 5 ELISA Kit | INSL5 elisa kit

Porcine Insulin Like Protein 5 ELISA Kit

Cat. Num. AAA742019

• Gene Names: INSL5; PRO182; UNQ156
• Reactivity: Porcine
• Synonyms: Insulin Like Protein 5; Porcine Insulin Like Protein 5 ELISA Kit; INSL5 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This INSL5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine INSL5. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INSL5 elisa kit

Principle of the Assay: INSL5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-INSL5 antibody and an INSL5-HRP conjugate. The assay sample and buffer are incubated together with INSL5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INSL5 concentration since INSL5 from samples and INSL5-HRP conjugate compete for the anti-INSL5 antibody binding site. Since the number of sites is limited, as more sites are occupied by INSL5 from the sample, fewer sites are left to bind INSL5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INSL5 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for INSL5 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 38569233
• NCBI GeneID: 10022
• NCBI Accession #: NP_005469.2
• NCBI GenBank Nucleotide #: NM_005478.4
• Molecular Weight: 15,333 Da
• NCBI Official Full Name: insulin-like peptide INSL5
• NCBI Official Synonym Full Names: insulin-like 5
• NCBI Official Symbol: INSL5
• NCBI Official Synonym Symbols: PRO182; UNQ156
• NCBI Protein Information: insulin-like peptide INSL5; prepro-INSL5; insulin-like peptide 5
• UniProt Protein Name: Insulin-like peptide INSL5
• Protein Family: Insulin-like peptide
• UniProt Gene Name: INSL5
• UniProt Synonym Gene Names: UNQ156/PRO182; Insulin-like peptide 5
• UniProt Entry Name: INSL5_HUMAN

NCBI Description

The protein encoded by this gene contains a classical signature of the insulin superfamily and is highly similar to relaxin 3 (RLN3/INSL7). [provided by RefSeq, Jul 2008]

Uniprot Description

INSL5: May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor. Belongs to the insulin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: extracellular region

Molecular Function: hormone activity