angiopoietin-2 Active Protein | Ang-2 active protein

Human angiopoietin-2 (Ang-2)

Cat. Num. AAA400087

• Gene Names: ANGPT2; ANG2; AGPT2
• Purity: The purity of Ang-2 Human is greater than 95.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Synonyms: angiopoietin-2; Human angiopoietin-2 (Ang-2); Ang-2 active protein

• Host: Insect Cells
• Purity/Purification: The purity of Ang-2 Human is greater than 95.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Form/Format: Sterile Filtered white lyophilized freeze dry powder.
• Sequence: The N-terminal sequence is that of myc
• Sequence Length: 444

• Solubility: It is recommended to reconstitute the lyophilized Angiopoitin-2 in sterile 18Momega-cm H2O not less than 100ug.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280 nm using the absorbency value of 1.40 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a standard solution of ANG-2 Human as a Reference Standard.
• Dimers and Aggregates: Less than 5%
• Formulation: Ang-2 Human is formulated from a solution containing TBS & 0.05% CHAPS.
• Biological Activity: The biological activity of Angiopoietin-2 was determined by the induction of endothelial cell sprouting as described in Korff et al., 2001.
• Preparation and Storage: Lyophilized Angiopoietin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Ang-2 Human Recombinant should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Please prevent freeze-thaw cycles.

Related Product Information for Ang-2 active protein

The Angiopoietins are a family of growth factors which bind to the endothelial receptor tyrosine kinase Tie2. Angiopoietin-1, 4 activate the Tie2 receptor, whereas Angiopoietin-2, 3 inhibit Angiopoietin-1-induced Tie2 phosphorylation. Ang-1 Human is a secreted growth factor which enhances endothelial cell survival and capillary morphogenesis, also it limits capillary permeability. Ang-2 is a natural inhibitor of Ang-1 because it binds the same receptor but fails activate it. When ambient levels of VEGF are high Angiopoietin-2 destabilizes capillary integrity, facilitating sprouting but when VEGF levels are low it causes vessel regression. Although Tie-1 and Tie-2 homologues but Tie-1's ligands are unknown. The mammalian metanephros (precursor of the adult kidney) is the local where Angiopoietin and Tie genes are expressed. These genes may play a role in endothelial precursor growth. When the metanephros first forms Tie-1-expressing cells can be detected. Ang-1 (podocyte-derived) and Ang-2 (mesangial-cell-derived) may affect growth of nascent capillaries during glomerular maturation. The descending limbs of loops of Henle in the mature vasa rectae (after birth) express Angiopoietin-2 which affects the growth of this medullary microcirculation. As gathered from recent data, angiopoietins are implicated in unregulated vessel growth in Wilms' kidney tumors and in vascular remodeling after nephrotoxicity. During vascular development VEGF-A and Angiopoietins not only have different roles, but also complementary and coordinated roles.
Angiopoietin-2 (Ang-2) Human, a 66 kDa protein consisting of 476 amino acid residues (N21-F496), is fused to a N-terminal myc-tag and produced in insect cells.

Product Categories/Family for Ang-2 active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 169646754
• NCBI GeneID: 285
• NCBI Accession #: NP_001112360.1
• NCBI GenBank Nucleotide #: NM_001118888.1
• UniProt Accession #: O15123; Q9NRR7; Q9P2Y7; A0AV38; A8K205; B7ZLM7
• Molecular Weight: 56,848 Da
• NCBI Official Full Name: angiopoietin-2 isoform c
• NCBI Official Synonym Full Names: angiopoietin 2
• NCBI Official Symbol: ANGPT2
• NCBI Official Synonym Symbols: ANG2; AGPT2
• NCBI Protein Information: angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a
• UniProt Protein Name: Angiopoietin-2
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT2
• UniProt Synonym Gene Names: ANG-2
• UniProt Entry Name: ANGP2_HUMAN

NCBI Description

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular space; cell projection; extracellular region; plasma membrane; nucleus

Molecular Function: protein binding; metal ion binding; receptor tyrosine kinase binding; receptor binding

Biological Process: Tie receptor signaling pathway; organ regeneration; maternal process involved in pregnancy; negative regulation of blood vessel endothelial cell migration; signal transduction; response to organic cyclic substance; germ cell development; negative regulation of positive chemotaxis; negative regulation of angiogenesis; response to radiation; positive regulation of angiogenesis; response to mechanical stimulus; response to glucose stimulus; response to hypoxia; angiogenesis; response to activity; blood coagulation; leukocyte migration