PDK1 active protein
Recombinant Human PDK1 (N-His tag)
Purity
70%-90%
Synonyms
PDK1; Recombinant Human PDK1 (N-His tag); PRO0461; PDPK1; MGC20087; MGC35290; PkB-like; PkB-like 1; PDK1 active protein
Host
Insect Cells
Purity/Purification
70%-90%
Form/Format
Liquid; 50mM NaPhosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol.
Sequence Length
129
Bioactivity
The specific activity of PDK1 was determined to be 118 nmol/min/mg as per activity assay protoco
Preparation and Storage
Store product at-70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Related Product Information for PDK1 active protein
PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3, 4, 5)P3 or PtdIns(3, 4)P2. PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns(3, 4, 5)P3 and/or PtdIns(3, 4)P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67.0 kDa
NCBI Official Full Name
PDK1, partial
Protein Family
Similar Products
Product Notes
The PDK1 (Catalog #AAA4160282) is an Active Protein produced from Insect Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PDK1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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