ADAM-17 active protein

ADAM-17 (catalytic domain) (human), (recombinant) (His-tag)

Cat. Num. AAA566602

• Purity: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Synonyms: ADAM-17; ADAM-17 (catalytic domain) (human); (recombinant) (His-tag); ADAM-17 active protein

• Host: Insect cells
• Purity/Purification: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Form/Format: Liquid. In 22.5mM TRIS, pH7.5, containing 4.5uM ZnCl2, 0.0045% Brij-35 and 10% glycerol

• Source Note: Produced in a baculovirus expression system. Recombinant glycosylated catalytic domain (aa Pro18-Val477) of ADAM17/TACE (A disintegrin and metalloproteinase 17; Tumor necrosis factor-alpha-converting enzyme), cloned from human cDNA (NM_003183), secreted as mature, active enzyme from insect cells, and purified using a C-terminal His-tag
• Specific Activity: >=1800U/ug enzyme. One unit will hydrolyze one pmole Mca-PLAQAV-Dpa-RSSSR-NH2 substrate (10uM) per minute at 37 degree C, in 25mM TRIS, pH9.0
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C; Salts (sodium chloride, calcium chloride, etc.) in the assay are inhibitory. ADAM17/TACE is stable after 6 freeze-thaws at ~0.4ug/ul; freeze-thaw stability of more dilute preparations has not been tested and could lead to loss of activity; Avoid freeze/thaw cycles; Shipping: Shipped on Dry Ice

Related Product Information for ADAM-17 active protein

ADAM17/TACE is a soluble or membrane-bound metalloproteinase primarily responsible for activation of proTNF-alpha, while also targeting proteins such as fractalkine, amyloid precursor proteins, and CD40. ADAM17/TACE is involved in cancer, vascular disorders, and inflammatory diseases such as rheumatoid arthritis and focal ischemic injury. The catalytic domain of ADAM17/TACE is able to cleave proTNF-alpha and can be used in inhibitor screening.

Product Categories/Family for ADAM-17 active protein

Cellular Analysis; Cell Organelles & Structures; Proteostasis; Proteolysis & Protein Degradation

NCBI and Uniprot Product Information

• UniProt Accession #: P78536
• Molecular Weight: ~305kDa (calculated); ~36kDa doublet (SDS-PAGE)
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 17
• UniProt Gene Name: ADAM17
• UniProt Synonym Gene Names: CSVP; TACE; ADAM 17
• UniProt Entry Name: ADA17_HUMAN

Uniprot Description

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 3.4.24.86; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: cell surface; focal adhesion; membrane; integral to plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; intercellular junction; actin cytoskeleton; lipid raft

Molecular Function: integrin binding; protein binding; interleukin-6 receptor binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; Notch binding; SH3 domain binding; PDZ domain binding

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; cell motility involved in cell locomotion; neutrophil mediated immunity; T cell differentiation in the thymus; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; extracellular matrix disassembly; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of interleukin-8 production; germinal center formation; cell adhesion; response to drug; spleen development; epidermal growth factor receptor signaling pathway; Notch signaling pathway; membrane protein intracellular domain proteolysis; response to high density lipoprotein stimulus; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; collagen catabolic process; regulation of mast cell apoptosis; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; wound healing, spreading of epidermal cells; positive regulation of cell migration

Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1