Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 (INPPL1) Recombinant Protein | INPPL1 recombinant protein

Recombinant Human Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 (INPPL1) , partial

Cat. Num. AAA1140625

• Gene Names: INPPL1; OPSMD; SHIP2
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Phosphatidylinositol 3; 4; 5-trisphosphate 5-phosphatase 2 (INPPL1); Recombinant Human Phosphatidylinositol 3; partial; INPPL1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,016

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 222136583
• NCBI GeneID: 3636
• NCBI Accession #: NP_001558.3
• NCBI GenBank Nucleotide #: NM_001567.3
• UniProt Accession #: O15357; Q13577; Q13578; B2RTX5
• Molecular Weight: 113,176 Da
• NCBI Official Full Name: phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2
• NCBI Official Synonym Full Names: inositol polyphosphate phosphatase like 1
• NCBI Official Symbol: INPPL1
• NCBI Official Synonym Symbols: OPSMD; SHIP2
• NCBI Protein Information: phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2
• UniProt Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2
• Protein Family: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase
• UniProt Gene Name: INPPL1
• UniProt Synonym Gene Names: SHIP2; INPPL-1; SH2 domain-containing inositol phosphatase 2; SHIP-2

NCBI Description

The protein encoded by this gene is an SH2-containing 5'-inositol phosphatase that is involved in the regulation of insulin function. The encoded protein also plays a role in the regulation of epidermal growth factor receptor turnover and actin remodelling. Additionally, this gene supports metastatic growth in breast cancer and is a valuable biomarker for breast cancer. [provided by RefSeq, Jan 2009]

Uniprot Description

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Plays a central role in regulation of PI3K-dependent insulin signaling, although the precise molecular mechanisms and signaling pathways remain unclear. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signaling or GLUT4 trafficking. Confers resistance to dietary obesity. May act by regulating AKT2, but not AKT1, phosphorylation at the plasma membrane. Part of a signaling pathway that regulates actin cytoskeleton remodeling. Required for the maintenance and dynamic remodeling of actin structures as well as in endocytosis, having a major impact on ligand-induced EGFR internalization and degradation. Participates in regulation of cortical and submembraneous actin by hydrolyzing PtdIns(3,4,5)P3 thereby regulating membrane ruffling (PubMed:21624956). Regulates cell adhesion and cell spreading. Required for HGF-mediated lamellipodium formation, cell scattering and spreading. Acts as a negative regulator of EPHA2 receptor endocytosis by inhibiting via PI3K-dependent Rac1 activation. Acts as a regulator of neuritogenesis by regulating PtdIns(3,4,5)P3 level and is required to form an initial protrusive pattern, and later, maintain proper neurite outgrowth. Acts as a negative regulator of the FC-gamma-RIIA receptor (FCGR2A). Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Involved in EGF signaling pathway. Upon stimulation by EGF, it is recruited by EGFR and dephosphorylates PtdIns(3,4,5)P3. Plays a negative role in regulating the PI3K-PKB pathway, possibly by inhibiting PKB activity. Down-regulates Fc-gamma-R-mediated phagocytosis in macrophages independently of INPP5D/SHIP1. In macrophages, down-regulates NF-kappa-B-dependent gene transcription by regulating macrophage colony-stimulating factor (M-CSF)-induced signaling. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. Involved in endochondral ossification.