INPP5A recombinant protein

Recombinant Human INPP5A Protein

Cat. Num. AAA8249686

• Gene Names: INPP5A; 5PTASE
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: INPP5A; Recombinant Human INPP5A Protein; Type I inositol 145-trisphosphate 5-phosphatase; 5PTase; INPP5A recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: MAGKAAAPGT AVLLVTANVG SLFDDPENLQ KNWLREFYQV VHTHKPHFMA LHCQEFGGKN YEASMSHVDK FVKELLSSDA MKEYNRARVY LDENYKSQEH FTALGSFYFL HESLKNIYQF DFKAKKYRKV AGKEIYSDTL ESTPMLEKEK FPQDYFPECK WSRKGFIRTR WCIADCAFDL VNIHLFHDAS NLVAWETSPS VYSGIRHKAL GYVLDRIIDQ RFEKVSYFVF GDFNFRLDSK SVVETLCTKA TMQTVRAADT NEVVKLIFRE SDNDRKVMLQ LEKKLFDYFN QEVFRDNNGT ALLEFDKELS VFKDRLYELD ISFPPSYPYS EDARQGEQYM NTRCPAWCDR ILMSPSAKEL VLRSESEEKV VTYDHIGPNV CMGDHKPVFL AFRIMPGAGK PHAHVHKCCV DHHHHHH
• Sequence Length: 348

• Applicable Applications for INPP5A recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for INPP5A recombinant protein

Recombinant Human INPP5A Protein is produced by our mammalian expression system and the target gene encoding Met1-Val410 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 1005261240
• NCBI GeneID: 3632
• NCBI Accession #: NP_001307971.1
• NCBI GenBank Nucleotide #: NM_001321042.1
• UniProt Accession #: Q14642; Q14640; Q5JSF1; D3DXI3
• Molecular Weight: 47,820 Da
• NCBI Official Full Name: type I inositol 1,4,5-trisphosphate 5-phosphatase isoform b
• NCBI Official Synonym Full Names: inositol polyphosphate-5-phosphatase A
• NCBI Official Symbol: INPP5A
• NCBI Official Synonym Symbols: 5PTASE
• NCBI Protein Information: type I inositol 1,4,5-trisphosphate 5-phosphatase
• UniProt Protein Name: Type I inositol 1,4,5-trisphosphate 5-phosphatase
• Protein Family: Type I inositol 1,4,5-trisphosphate 5-phosphatase
• UniProt Gene Name: INPP5A
• UniProt Synonym Gene Names: 5PTase
• UniProt Entry Name: I5P1_HUMAN

NCBI Description

The protein encoded by this gene is a membrane-associated type I inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. [provided by RefSeq, Jul 2008]

Uniprot Description

INPP5A: Major isoenzyme hydrolyzing the calcium-mobilizing second messenger Ins(1,4,5)P3, this is a signal-terminating reaction. Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type I family.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 3.1.3.56; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: intracellular; plasma membrane

Molecular Function: inositol-polyphosphate 5-phosphatase activity; PH domain binding; protein binding

Biological Process: inositol phosphate dephosphorylation; inositol phosphate metabolic process; inositol phosphate-mediated signaling