WB (Western Blot)
(Detection of human Neurofilament-L by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, HeLa, SK-N-BE(2), Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
Neurofilament-L Recombinant Antibody | NEFL recombinant antibody
Rabbit anti-Neurofilament-L Recombinant Monoclonal Antibody [BLR224K]
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Neurofilament-L by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, HeLa, SK-N-BE(2), Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human Neurofilament-L by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, HeLa, SK-N-BE(2), Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse and rat Neurofilament-L by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, B35, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of mouse and rat Neurofilament-L by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, B35, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human Neurofilament-L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874 lot 1) used for IP at 20 ul/mg lysate. Neurofilament-L was also immunoprecipitated by a second antibody against a different epitope of Neurofilament-L (BL-2033B-1C7). For blotting immunoprecipitated Neurofilament-L, AAA23874 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Neurofilament-L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874 lot 1) used for IP at 20 ul/mg lysate. Neurofilament-L was also immunoprecipitated by a second antibody against a different epitope of Neurofilament-L (BL-2033B-1C7). For blotting immunoprecipitated Neurofilament-L, AAA23874 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human Neurofilament-L by immunohistochemistry. Sample: FFPE section of human medulla. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human Neurofilament-L by immunohistochemistry. Sample: FFPE section of human medulla. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of mouse Neurofilament-L by immunohistochemistry. Sample: FFPE section of mouse cerebellum. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of mouse Neurofilament-L by immunohistochemistry. Sample: FFPE section of mouse cerebellum. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of rat Neurofilament-L by immunocytochemistry. Sample: FFPE section of B35 cells. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of rat Neurofilament-L by immunocytochemistry. Sample: FFPE section of B35 cells. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human Neurofilament-L by immunocytochemistry. Sample: FFPE section of SK-N-BE(2) cells. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human Neurofilament-L by immunocytochemistry. Sample: FFPE section of SK-N-BE(2) cells. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
NCBI and Uniprot Product Information
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Product Notes
The NEFL nefl (Catalog #AAA23874) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Neurofilament-L Recombinant Monoclonal Antibody [BLR224K] reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament-L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Researchers should empirically determine the suitability of the NEFL nefl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament-L, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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