AZD9291, inhibitor
AZD9291
Purity
98.0%
Synonyms
AZD9291; inhibitor
Purity/Purification
98.0%
Form/Format
POWDER
Application Notes
Cell Assay: AZD-9291 is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. PC-9 cells are seeded into T75 flasks (5×105 cells/flask) in RPMI growth media and incubated at 37°C, 5% CO2. The following day the media is replaced with media supplemented with a concentration of EGFR inhibitor equal to the EC50 concentration predetermined in PC-9 cells. Media changes are carried out every 2-3 days and resistant clones allowed to grow to 80% confluency prior to the cells being trypsinised and reseeded at the original seeding density in media containing twice the concentration of EGFR inhibitor. Dose escalations are continued until a final concentration of 1.5 uM Gefitinib, 1.5 uM Afatinib, 1.5 uM WZ4002 or 160 nM AZD-9291 are achieved[1].
Reference:
1. Patent. 2013, WO2013014448 A1.
2. Cross, et al. Mol Cancer Ther. 2013, 12, A109.
Reference:
1. Patent. 2013, WO2013014448 A1.
2. Cross, et al. Mol Cancer Ther. 2013, 12, A109.
CAS Number
1421373-65-0
Molecular Formula
C28H33N7O2
Solubility
DMSO: 92 mg/mL (184.1 mM)
Ethanol: 22 mg/mL (44 mM)
Water: < 1 mg/mL
Ethanol: 22 mg/mL (44 mM)
Water: < 1 mg/mL
Receptor (IC50)
EGFR (Exon 19 deletion): 12.92nM
EGFR (L858R/T790M): 1nM
EGFR (L858R/T790M): 1nM
Animal Experiment
Animal Model: Mice
Smiles
c1cnc(nc1c1cn(c2c1cccc2)C)Nc1c(cc(c(c1)NC(=O)C=C)N(CCN(C)C)C)OC
Preparation and Storage
2 years -80°C in solvent
3 years -20°C in powder
3 years -20°C in powder
Related Product Information for AZD9291, inhibitor
Osimertinib is a small molecule tyrosine kinase receptor inhibitor and antineoplastic agent that is used in the therapy of selected forms of advanced non-small cell lung cancer (NSCLC).
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Product Notes
The AZD9291 (Catalog #AAA576635) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. Cell Assay: AZD-9291 is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. PC-9 cells are seeded into T75 flasks (5×105 cells/flask) in RPMI growth media and incubated at 37°C, 5% CO2. The following day the media is replaced with media supplemented with a concentration of EGFR inhibitor equal to the EC50 concentration predetermined in PC-9 cells. Media changes are carried out every 2-3 days and resistant clones allowed to grow to 80% confluency prior to the cells being trypsinised and reseeded at the original seeding density in media containing twice the concentration of EGFR inhibitor. Dose escalations are continued until a final concentration of 1.5 uM Gefitinib, 1.5 uM Afatinib, 1.5 uM WZ4002 or 160 nM AZD-9291 are achieved[1]. Reference: 1. Patent. 2013, WO2013014448 A1. 2. Cross, et al. Mol Cancer Ther. 2013, 12, A109. Researchers should empirically determine the suitability of the AZD9291 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AZD9291, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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