IMPDH2 recombinant protein

IMPDH2, 1-514aa, Human, His tag, E Coli

Cat. Num. AAA204353

• Gene Names: IMPDH2; IMPD2; IMPDH-II
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: IMPDH2; 1-514aa; Human; His tag; E Coli; Inosine-5'-monophosphate dehydrogenase 2; IMPD2; IMPDH-II; IMPDH2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MADYLISGGT SYVPDDGLTA QQLFNCGDGL TYNDFLILPG YIDFTADQVD LTSALTKKIT LKTPLVSSPM DTVTEAGMAI AMALTGGIGF IHHNCTPEFQ ANEVRKVKKY EQGFITDPVV LSPKDRVRDV FEAKARHGFC GIPITDTGRM GSRLVGIISS RDIDFLKEEE HDCFLEEIMT KREDLVVAPA GITLKEANEI LQRSKKGKLP IVNEDDELVA IIARTDLKKN RDYPLASKDA KKQLLCGAAI GTHEDDKYRL DLLAQAGVDV VVLDSSQGNS IFQINMIKYI KDKYPNLQVI GGNVVTAAQA KNLIDAGVDA LRVGMGSGSI CITQEVLACG RPQATAVYKV SEYARRFGVP VIADGGIQNV GHIAKALALG ASTVMMGSLL AATTEAPGEY FFSDGIRLKK YRGMGSLDAM DKHLSSQNRY FSEADKIKVA QGVSGAVQDK GSIHKFVPYL IAGIQHSCQD IGAKSLTQVR AMMYSGELKF EKRTSSAQVE GGVHSLHSYE KRLF
• Sequence Length: 514

• Applicable Applications for IMPDH2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for IMPDH2 recombinant protein

IMPDH2 belongs to the IMPDH/GMPR family. It catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. IMPDH2 is the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. Also IMPDH1 and IMPDH2 are targets for the important immunosuppressive drug, mycophenolic acid (MPA). Recombinant human IMPDH2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for IMPDH2 recombinant protein

Metabolism

References

Wu TY, Peng Y, et al. (2010) Pharmacol. 161 (7):1584-98. ; Bremer S, et al. (2008) Transplantation. 85 (1):55-61.

NCBI and Uniprot Product Information

• NCBI GI #: 66933016
• NCBI GeneID: 3615
• NCBI Accession #: NP_000875
• NCBI GenBank Nucleotide #: NM_000884.2
• UniProt Accession #: P12268; Q6LEF3
• Molecular Weight: 58.0kDa (534aa)
• NCBI Official Full Name: inosine-5'-monophosphate dehydrogenase 2
• NCBI Official Synonym Full Names: inosine monophosphate dehydrogenase 2
• NCBI Official Symbol: IMPDH2
• NCBI Official Synonym Symbols: IMPD2; IMPDH-II
• NCBI Protein Information: inosine-5'-monophosphate dehydrogenase 2
• UniProt Protein Name: Inosine-5'-monophosphate dehydrogenase 2
• UniProt Gene Name: IMPDH2
• UniProt Synonym Gene Names: IMP dehydrogenase 2; IMPD 2; IMPDH 2

NCBI Description

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.