Human IMP (inosine monophosphate) dehydrogenase 2 ELISA Kit | IMPDH2 elisa kit

Human Inosine-5'-monophosphate dehydrogenase 2, IMPDH2 ELISA Kit

Cat. Num. AAA906235

• Gene Names: IMPDH2; IMPD2; IMPDH-II
• Reactivity: Human
• Synonyms: IMP (inosine monophosphate) dehydrogenase 2; Human Inosine-5'-monophosphate dehydrogenase 2; IMPDH2 ELISA Kit; Human Inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) ELISA kit; hCG_2002013; IMPD2; IMPDH-II; IMP oxireductase 2; inosine 5 phosphate dehydrogenase 2; inosine monophosphate dehydrogenase 2; inosine monophosphate dehydrogenase type II; IMPDH2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human IMPDH2. No significant cross-reactivity or interference between Human IMPDH2 and analogues was observed.
• Sequence Length: 514

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 23.5 pg/ml-1500 pg/ml
• Sensitivity: 5.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IMPDH2 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IMPDH2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IMPDH2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IMPDH2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IMPDH2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 66933016
• NCBI GeneID: 3615
• NCBI Accession #: NP_000875.2
• NCBI GenBank Nucleotide #: NM_000884.2
• UniProt Accession #: P12268; Q6LEF3
• Molecular Weight: 55,805 Da
• NCBI Official Full Name: inosine-5'-monophosphate dehydrogenase 2
• NCBI Official Synonym Full Names: IMP (inosine 5'-monophosphate) dehydrogenase 2
• NCBI Official Symbol: IMPDH2
• NCBI Official Synonym Symbols: IMPD2; IMPDH-II
• NCBI Protein Information: inosine-5'-monophosphate dehydrogenase 2; IMPD 2; IMPDH 2; IMP oxireductase 2; inosine 5' phosphate dehydrogenase 2; IMP (inosine monophosphate) dehydrogenase 2; inosine monophosphate dehydrogenase type II
• UniProt Protein Name: Inosine-5'-monophosphate dehydrogenase 2
• UniProt Gene Name: IMPDH2
• UniProt Synonym Gene Names: IMPD2; IMP dehydrogenase 2; IMPD 2; IMPDH 2
• UniProt Entry Name: IMDH2_HUMAN

NCBI Description

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq, Jul 2008]

Uniprot Description

IMPDH2: a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.

Protein type: Nucleotide Metabolism - purine; Oxidoreductase; EC 1.1.1.205; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 3p21.2

Cellular Component: peroxisomal membrane; membrane; cytoplasm; cytosol; nucleus

Molecular Function: DNA binding; RNA binding; metal ion binding; nucleotide binding; IMP dehydrogenase activity

Biological Process: lymphocyte proliferation; purine ribonucleoside monophosphate biosynthetic process; retina development in camera-type eye; nucleobase, nucleoside and nucleotide metabolic process; GMP biosynthetic process; purine base metabolic process; protein homotetramerization