Glycoprotein 130 (gp130) Recombinant Protein | gp130 recombinant protein

Recombinant Glycoprotein 130 (gp130)

Cat. Num. AAA2011690

• Gene Names: IL6ST; CD130; GP130; CDW130; IL-6RB
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Glycoprotein 130 (gp130); Recombinant Glycoprotein 130 (gp130); gp130 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-TNDRYLATL TVRNLVGKSD AAVLTIPACD FQGNLAESKC YLITVTPVYA DGPGSPESIK AYLKQAPPSK GPTVRTKKVG KNEAVLEWDQ LPVDVQNGFI RNYTIFYRTI IGNETAVNVD SSHTEYTLSS LTSDTLYMVR MAAYTDEGGK DGPEFTFTTP KFAQGEIEAI VVPVCL
• Sequence Length: 857

• Applicable Applications for gp130 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Thr392~Leu566 (Accession # P40189) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 300244535
• NCBI GeneID: 3572
• NCBI Accession #: NP_001177910.1
• NCBI GenBank Nucleotide #: NM_001190981.1
• UniProt Accession #: P40189; Q5FC04; Q9UQ41; A0N0L4
• Molecular Weight: 20.3kDa
• NCBI Official Full Name: interleukin-6 receptor subunit beta isoform 3
• NCBI Official Synonym Full Names: interleukin 6 signal transducer (gp130, oncostatin M receptor)
• NCBI Official Symbol: IL6ST
• NCBI Official Synonym Symbols: CD130; GP130; CDW130; IL-6RB
• NCBI Protein Information: interleukin-6 receptor subunit beta; CD130 antigen; IL-6R subunit beta; membrane glycoprotein 130; IL-6 receptor subunit beta; membrane glycoprotein gp130; interleukin receptor beta chain; oncostatin-M receptor subunit alpha; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
• UniProt Protein Name: Interleukin-6 receptor subunit beta
• UniProt Gene Name: IL6ST
• UniProt Synonym Gene Names: IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; gp130
• UniProt Entry Name: IL6RB_HUMAN

NCBI Description

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]

Uniprot Description

gp130: gp130 is a ubiquitously expressed type I cytokine family receptor. The receptor systems for IL6, LIF, OSM, CNTF, IL11 AND CT1 utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not directly bind IL6. May have a role in embryonic development. Contains 5 fibronectin type III domains and 1 immunoglobulin-like C2-type domain. Two alternatively spliced isoforms have been described.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular space; cell soma; membrane; oncostatin-M receptor complex; dendrite; extracellular region; plasma membrane; interleukin-6 receptor complex; external side of plasma membrane

Molecular Function: interleukin-11 binding; interleukin-27 receptor activity; protein homodimerization activity; leukemia inhibitory factor receptor activity; oncostatin-M receptor activity; interleukin-6 receptor activity; interleukin-11 receptor activity; protein binding; interleukin-6 binding; interleukin-6 receptor binding; growth factor binding; ciliary neurotrophic factor receptor activity; ciliary neurotrophic factor receptor binding

Biological Process: glycogen metabolic process; viral reproduction; leukemia inhibitory factor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of osteoblast differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; regulation of Notch signaling pathway; positive regulation of acute inflammatory response; response to cytokine stimulus; positive regulation of cell proliferation; positive regulation of T cell proliferation; positive regulation of adaptive immune response; negative regulation of apoptosis; positive regulation of astrocyte differentiation