IL5 recombinant protein

Recombinant Human IL5 protein with C-terminal 6xHis tag

Cat. Num. AAA188123

• Gene Names: IL5; EDF; TRF; IL-5
• Applications: Western Blot
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Synonyms: IL5; Recombinant Human IL5 protein with C-terminal 6xHis tag; Human IL5 Protein; His Tag; IL-5; TRF; Interleukin-5; EDF; IL5 recombinant protein

• Host: HEK293 cells
• Purity/Purification: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form/Format: Lyophilized from sterile PBS, pH 7.4. Normally 5%-72% trehalose is added as protectants before lyophilization.
• Sequence: IL5(Ile20-Ser134)

• Applicable Applications for IL5 recombinant protein: Western Blot (WB)
• Source: Human
• Conjugation: C-6xHis tag
• Reconstitution: Reconstitute with deionized water
• Preparation and Storage: Store at -80 degree C for 12 months (Avoid repeated freezing and thawing); Shipping: Blue Ice

SDS-Page

(Figure 1. Human IL5 Protein, His Tag on SDS-PAGE under reducing condition.)

Related Product Information for IL5 recombinant protein

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2. This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

NCBI and Uniprot Product Information

• NCBI GI #: 4504671
• NCBI GeneID: 3567
• NCBI Accession #: NP_000870.1
• NCBI GenBank Nucleotide #: NM_000879.2
• UniProt Accession #: P05113; Q13840
• Molecular Weight: 15,238 Da
• NCBI Official Full Name: interleukin-5
• NCBI Official Synonym Full Names: interleukin 5
• NCBI Official Symbol: IL5
• NCBI Official Synonym Symbols: EDF; TRF; IL-5
• NCBI Protein Information: interleukin-5; B-cell differentiation factor I; T-cell replacing factor; colony-stimulating factor, eosinophil; eosinophil differentiation factor
• UniProt Protein Name: Interleukin-5
• Protein Family: Interleukin
• UniProt Gene Name: IL5
• UniProt Synonym Gene Names: IL-5; TRF
• UniProt Entry Name: IL5_HUMAN

NCBI Description

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Uniprot Description

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of transcription factor activity; immune response; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation