CXCL10 recombinant protein

Human CXCL10 Protein

Cat. Num. AAA9718691

• Gene Names: IL36RN; FIL1; FIL1D; IL1F5; IL1L1; PSORP; IL1HY1; IL1RP3; IL36RA; IL-36Ra; PSORS14; FIL1(DELTA)
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CXCL10; Human CXCL10 Protein; Recombinant Human CXCL10 Protein (Interleukin-36 receptor antagonist protein) expressed in E. coli; C7; crg-2; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10; Interleukin-36 receptor antagonist protein; CXCL10 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS
• Sequence: Amino acid sequence derived from mature form of human CXCL10 (Q9UBH0) (Val22-Pro98) was expressed, with a N-terminal Met.
• Sequence Length: 155

• Species: Human
• Protein Length: The recombinant human CXCL10 consists of 78 amino acids and predicts a molecular mass of 8.8 KDa. It migrates as an approximately 9 KDa band in SDS-PAGE under reducing conditions.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CXCL10 protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human CXCL10 protein.)

Related Product Information for CXCL10 recombinant protein

CXCL10, also known as crg-2, is a chemokine of the CXC subfamily and ligand for the receptor CXCR3. CXC chemokines are particularly significant for leukocyte infiltration in inflammatory diseases. CXCL10 has a three-dimensional crystal structure. Its signaling is mediated by the g protein-coupled receptor CXCR3, which is expressed on activated T cells and plays an important role in directing the migration of T cells, especially during Th1 responses. Binding of CXCL10 to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. It is chemotactic for monocytes and T-lymphocytes. CXCL10 can be secreted by several cell types in response to IFN-gamma. Baseline pre-treatment plasma levels of CXCL10 are elevated in patients chronically infected with hepatitis C virus (HCV) of genotypes 1 or 4 who do not achieve a sustained viral response (SVR) after completion of antiviral therapy.

NCBI and Uniprot Product Information

• NCBI GI #: 6912432
• NCBI GeneID: 26525
• NCBI Accession #: NP_036407.1
• NCBI GenBank Nucleotide #: NP_036407.1
• UniProt Accession #: Q9UBH0
• Molecular Weight: 8.8 kDa
• NCBI Official Full Name: interleukin-36 receptor antagonist protein
• NCBI Official Synonym Full Names: interleukin 36 receptor antagonist
• NCBI Official Symbol: IL36RN
• NCBI Official Synonym Symbols: FIL1; FIL1D; IL1F5; IL1L1; PSORP; IL1HY1; IL1RP3; IL36RA; IL-36Ra; PSORS14; FIL1(DELTA)
• NCBI Protein Information: interleukin-36 receptor antagonist protein
• UniProt Protein Name: Interleukin-36 receptor antagonist protein
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: IL36RN
• UniProt Synonym Gene Names: FIL1D; IL1F5; IL1HY1; IL1L1; IL1RP3; IL-1RP3; IL-1HY1; IL-1 delta; IL-1F5; IL-1ra homolog 1; IL-1L1
• UniProt Entry Name: I36RA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1F5: Is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL- 1R1), that is present in epithelial barriers and takes part in local inflammatory response. Defects in IL36RN are the cause of psoriasis generalized pustular (PSORP). PSORP is a life-threatening disease defined by repeated flares of sudden onset consisting of diffuse erythematous skin eruption characterized by rapid coverage with pustules, high-grade fever, asthenia, marked leukocytosis, and elevated serum levels of C-reactive protein. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space

Molecular Function: cytokine activity; interleukin-1 receptor binding; protein binding

Biological Process: antifungal humoral response; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of interleukin-17 production

Disease: Psoriasis 14, Pustular