Interleukin-24 Recombinant Protein | IL-24 recombinant protein

Human Interleukin-24 Recombinant

Cat. Num. AAA553230

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-24; Human Interleukin-24 Recombinant; MDA7; ST16; il24; interleukin-24; il-24; IL-24 recombinant protein

• Host: HEk293 Cells
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Interleukin-24 was lyophilized from 0.2um filtered solution in PBS, pH 7.4 including 5% (w/v) trehalose.
• Sequence: MNFQQRLQSL WTLARPFCPP LLATASQMQM VVLPCLGFTL LLWSQVSGAQ GQEFHFGPCQ VKGVVPQKLW EAFWAVKDTM QAQDNITSAR LLQQEVLQNV SDAESCYLVH TLLEFYLKTV FKNYHNRTVE VRTLKSFSTL ANNFVLIVSQ LQPSQENEMF SIRDSAHRRF LLFRRAFKQL DVEAALTKAL GEVDILLTWM QKFYKL
• Sequence Length: 207

• Domain: 4 Helix Cytokine
• Host Note: Optimized DNA sequence encoding Human Interleukin-24 mature chain including a C-terminal His tag was expressed in HEK293 cells.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human IL-24 has a calculated molecular mass of approximately 18.5 kDa. ; ; Recombinant IL-24 is a disulfide-linked monomeric protein consisting of 165 amino acid residue subunits, and migrates as an approximately 35kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
• Preparation and Storage: Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-24 recombinant protein

IL-24 (melanoma differentiation-associated gene-7 (mda-7)), a member of the IL-10 cytokine family, possesses the properties of a classical cytokine as well as tumor suppressor effects. The exact role of IL-24 in the immune system has not been defined but studies have indicated a role for IL-24 in inflammatory conditions such as psoriasis. The tumor suppressor effects of IL-24 include inhibition of angiogenesis, sensitization to chemotherapy, and p38 mitogen-activated protein kinase (MAPK) -mediated apoptosis. Current knowledge on the regulation of IL-24 expression is sparse. The melanoma differentiation- associated gene-7 (mda-7) was discovered through subtraction hybridization of cDNA libraries prepared from melanoma cells. As mda-7 shares considerable structural and sequence homology with IL-10 cytokine family members it was recognized as a member of this family and renamed interleukin-24 (IL-24). IL-24 signals through a heterodimeric receptor complex consisting of the IL-20Rβ subunit together with either the IL-20Rα subunit or the IL-22Rα subunit. The IL-20Rα/IL-20Rβ complex is shared with IL-19 and IL-20 whereas the IL- 22Rα/IL-20Rβ complex is shared with only IL-20. Binding of IL-24 as well as IL-19 and IL-20 to the receptor complexes results in signal transducer and activator of transcription 3 (STAT3) -activation.

Product Categories/Family for IL-24 recombinant protein

Cytokines

References

• a serotype 5/3 adenovirus expressing mda-7/IL-24 infects renal carcinoma cells and promotes toxicity of agents that increase ros and ceramide levels .Mol. Pharmacol., Nov 2010; 10.1124/mol.110.069484.
• a single intramuscular injection of aav-8 vector expressing mda7/IL-24 efficiently suppresses tumor growth mediated by multiple anti-cancer mechanisms in lymphoma model mice. .Blood (ASH Annual Meeting Abstracts), Nov 2010; 116: 1474.
• mechanism by which mcl-1 regulates cancer-specific apoptosis triggered by mda-7/IL-24, an il-10–related cytokine .Cancer Res., Jun 2010; 70: 5034 - 5045.
• IL-24 transgenic mice: in vivo evidence of overlapping functions for il-20, il-22, and IL-24 in the epidermis .J. Immunol., Feb 2010; 184: 1793 - 1798.
• perk–dependent regulation of ceramide synthase 6 and thioredoxin play a key role in mda-7/IL-24–induced killing of primary human glioblastoma multiforme cells .Cancer Res., Feb 2010; 70: 1120 - 1129.