Human interleukin 1 family, member 5 (delta) ELISA Kit | IL1F5 elisa kit

Human Interleukin-36 receptor antagonist protein, IL1F5 ELISA Kit

Cat. Num. AAA914111

• Gene Names: IL36RN; FIL1; FIL1D; IL1F5; IL1L1; PSORP; IL1HY1; IL1RP3; IL36RA; FIL1(DELTA)
• Reactivity: Human
• Synonyms: interleukin 1 family; member 5 (delta); Human Interleukin-36 receptor antagonist protein; IL1F5 ELISA Kit; Human Interleukin-36 receptor antagonist protein (IL1F5) ELISA kit; FIL1; FIL1 (DELTA); FIL1D; IL1HY1; IL1L1; IL1RP3; MGC29840; IL-1 related protein 3; IL-1F5 (IL-1HY1; FIL1-delta; IL-1RP3; IL-1L1; IL-1-delta) ; IL-1ra homolog; IL1F5 (Canonical product IL-1F5a) ; family of; IL1F5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human IL1F5. No significant cross-reactivity or interference between human IL1F5 and analogues was observed.
• Sequence Length: 155

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 7.8 pg/ml-500 pg/ml
• Sensitivity: < 1.95 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL1F5 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL1F5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1F5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1F5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1F5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6912432
• NCBI GeneID: 26525
• NCBI Accession #: NP_036407.1
• NCBI GenBank Nucleotide #: NM_012275.2
• UniProt Accession #: Q9UBH0; Q56AT9; Q7RTZ6; A8K2I4
• Molecular Weight: 16,962 Da
• NCBI Official Full Name: interleukin-36 receptor antagonist protein
• NCBI Official Synonym Full Names: interleukin 36 receptor antagonist
• NCBI Official Symbol: IL36RN
• NCBI Official Synonym Symbols: FIL1; FIL1D; IL1F5; IL1L1; PSORP; IL1HY1; IL1RP3; IL36RA; FIL1(DELTA)
• NCBI Protein Information: interleukin-36 receptor antagonist protein; IL-1ra homolog 1; interleukin-1 HY1; IL-1 related protein 3; interleukin-1-like protein 1; IL1F5 (Canonical product IL-1F5a); interleukin 1 family, member 5 (delta); interleukin-1 receptor antagonist homolog 1; IL-1F5 (IL-1HY1, FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta)
• UniProt Protein Name: Interleukin-36 receptor antagonist protein
• UniProt Gene Name: IL36RN
• UniProt Synonym Gene Names: FIL1D; IL1F5; IL1HY1; IL1L1; IL1RP3; UNQ1896/PRO4342; IL-1RP3; IL-1HY1; IL-1 delta; IL-1F5; IL-1ra homolog 1; IL-1L1
• UniProt Entry Name: I36RA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1F5: Is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL- 1R1), that is present in epithelial barriers and takes part in local inflammatory response. Defects in IL36RN are the cause of psoriasis generalized pustular (PSORP). PSORP is a life-threatening disease defined by repeated flares of sudden onset consisting of diffuse erythematous skin eruption characterized by rapid coverage with pustules, high-grade fever, asthenia, marked leukocytosis, and elevated serum levels of C-reactive protein. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space

Molecular Function: interleukin-1 receptor binding; cytokine activity; interleukin-1 receptor antagonist activity

Biological Process: negative regulation of interleukin-17 production; cytokine and chemokine mediated signaling pathway; negative regulation of interleukin-6 production; antifungal humoral response; innate immune response; negative regulation of cytokine and chemokine mediated signaling pathway

Disease: Psoriasis 14, Pustular