IL-17RA (CD217) Fc tagged Recombinant Protein | IL17RA recombinant protein

Human IL-17RA (CD217) Fc tagged Recombinant

Cat. Num. AAA553248

• Reactivity: Measured by its ability to bind immobilized recombinant human IL-17A at a concentration range of 1-32ng/ml in a functional ELISA assay.
• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: IL-17RA (CD217) Fc tagged; Human IL-17RA (CD217) Fc tagged Recombinant; Human IL-17 receptor Fc tag Recombinant; il-17ra; il17RA; interleukin-17 r; IL17RA recombinant protein

• Host: HEk293 Cells
• Reactivity: Measured by its ability to bind immobilized recombinant human IL-17A at a concentration range of 1-32ng/ml in a functional ELISA assay.
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Interleukin-17 receptor alpha is supplied as lyophilized 0.2 um filtered PBS solution, pH7.2.
• Sequence Length: 866

• Host Note: Optimized DNA sequence encoding extracellular domain of human IL17R receptor including a C terminal Human IgG1 Fc tag was expressed in HEK293 cells.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Molecular Weight Note: Recombinant IL17RA/Fc is a homodimer protein consisting of 300 amino acid residue subunits, due to glycosylation migrates as an approximately 55kD protein under reducing SDS-PAGE conditions.
• Preparation and Storage: Recombinant IL-17 receptor alpha, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months.; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL17RA recombinant protein

IL-17 is a potent regulator of the neutrophil response. Both IL-17A and IL-17F are capable of signaling through IL-17RA to induce granulopoietic factors such as G-CSF and stem cell factor which leads to expansion of neutrophil progenitors in the bone marrow and spleen, as well as expansion of mature neutrophils in peripheral blood. IL-17RA signaling up- regulates production of neutrophilic chemokines such as CXCL1 (KC), CXCL2, CXCL5, CXCL6, and CXCL8, which in turn regulate neutrophil emigration to mucosal sites. Due to this role in neutrophil expansion and recruitment, IL-17RA signaling is an important part of the host response to extracellular bacterial infections.To date, four additional receptors have been identified in the IL- 17R family based on sequence homology to IL-17R (IL- 17Rh1, IL-17RC, IL-17RD, and IL-17RE) and among them, IL- 17RC has been shown recently to physically associate with IL-17RA, suggesting that it may be a functional component in the IL-17R complex. Nevertheless, the final structure of the functional IL-17 receptor and the mechanisms of interactions with IL-17A remain unknown.

Product Categories/Family for IL17RA recombinant protein

Receptors CD

References

• cloning and characterisation of rainbow trout interleukin (il) -17a/f2 and IL-17RA: expression during infection and bioactivity of the recombinant il-17a/f2. .Infect. Immun., Nov 2012; 10.1128/IAI.00599-12.
• the il-17a/IL-17RA axis plays a proatherogenic role via the regulation of aortic myeloid cell recruitment .Circ. Res., Mar 2012; 110: 675 - 687.
• a cc' loop decoy peptide blocks the interaction between act1 and IL-17RA to attenuate il-17– and il-25–induced inflammation .Sci. Signal., Nov 2011; 4: ra72.
• lack of IL-17RA signalling prevents autoimmune inflammation of the joint and give rise to a th2-like phenotype in collagen-induced arthritis .Ann Rheum Dis, Mar 2011; 70: A50 - A51.
• il-17a- versus il-17f-induced intracellular signal transduction pathways and modulation by IL-17RA and il-17rc rna interference in rheumatoid synoviocytes .Ann Rheum Dis, Feb 2011; 70: 341 - 348.