Mouse interleukin 17A ELISA Kit | IL17A elisa kit

Mouse Interleukin 17, IL-17 ELISA Kit

Cat. Num. AAA701528

• Gene Names: Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
• Reactivity: Mouse
• Synonyms: interleukin 17A; Mouse Interleukin 17; IL-17 ELISA Kit; CTLA8; IL-17; IL-17A; IL17; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; cytotoxic T-lymphocyte-associated serine esterase 8; interleukin 17 (cytotoxic T-ly; IL17A elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse IL17A. No significant cross-reactivity or interference between Mouse IL17A and analogues was observed.
• Sequence Length: 158

• Samples: Serum, plasma, tissue homogenates and cell culture supernates
• Detection Range: 47 pg/ml-3000 pg/ml
• Sensitivity: 11.75 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL17A elisa kit

Introduction: Interleukin-17 (IL-17, or IL-17A) is the founding member of a group of cytokines called the IL-17 family. IL-17A, was originally identified as a transcript from a rodent T-cell hybridoma by Rouvier et al. in 1993. Known as CTLA8 in rodents, IL-17 shows high homology to viral IL-17 encoded by an open reading frame of the T lymphotropic rhadinovirus Herpesvirus saimiri.[1] To elicit its functions, IL-17 binds to a type I cell surface receptor called IL-17R of which there are at least three variants IL17RA, IL17RB, and IL17RC.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-17. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-17 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-17 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6754324
• NCBI GeneID: 16171
• NCBI Accession #: NP_034682.1
• NCBI GenBank Nucleotide #: NM_010552.3
• UniProt Accession #: Q62386; Q60971
• Molecular Weight: 17,490 Da
• NCBI Official Full Name: interleukin-17A
• NCBI Official Synonym Full Names: interleukin 17A
• NCBI Official Symbol: Il17a
• NCBI Official Synonym Symbols: Il17; Ctla8; IL-17; Ctla-8; IL-17A
• NCBI Protein Information: interleukin-17A; cytotoxic T-lymphocyte-associated antigen 8
• UniProt Protein Name: Interleukin-17A
• Protein Family: Interleukin
• UniProt Gene Name: Il17a
• UniProt Synonym Gene Names: Ctla8; Il17; IL-17; IL-17A; CTLA-8
• UniProt Entry Name: IL17_MOUSE

Uniprot Description

Function: Induces stromal cells to produce proinflammatory and hematopoietic cytokines