Interleukin 12 Receptor Beta 1 (IL12Rb1) Recombinant Protein | IL12Rb1 recombinant protein

Recombinant Interleukin 12 Receptor Beta 1 (IL12Rb1)

Cat. Num. AAA2010022

• Gene Names: IL12RB1; CD212; IL12RB; IL-12R-BETA1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interleukin 12 Receptor Beta 1 (IL12Rb1); Recombinant Interleukin 12 Receptor Beta 1 (IL12Rb1); IL12Rb1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-LTLW STVLSTYHFG GNASAAGTPH HVSVKNHSLD SVSVDWAPSL LSTCPGVLKE YVVRCRDEDS KQVSEHPVQP TETQVTLSGL RAGVAYTVQV RADTAWLRGV WSQPQRFSIE VQVSDWLIFF ASLGSFLSIL LVGVLGYLGL NRAARHLCPP LPTPCASSAI EFPGGKETWQ WIN
• Sequence Length: 662

• Applicable Applications for IL12Rb1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Leu429~Asn603 (Accession # P42701) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5031785
• NCBI GeneID: 3594
• NCBI Accession #: NP_005526.1
• NCBI GenBank Nucleotide #: NM_005535.1
• UniProt Accession #: P42701; Q8N6Q7; A8K308; B2RPF1; B7ZKK3
• Molecular Weight: 20.6kDa
• NCBI Official Full Name: interleukin-12 receptor subunit beta-1 isoform 1
• NCBI Official Synonym Full Names: interleukin 12 receptor, beta 1
• NCBI Official Symbol: IL12RB1
• NCBI Official Synonym Symbols: CD212; IL12RB; IL-12R-BETA1
• NCBI Protein Information: interleukin-12 receptor subunit beta-1; IL-12R subunit beta-1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; interleukin-12 receptor beta-1 chain
• UniProt Protein Name: Interleukin-12 receptor subunit beta-1
• Protein Family: Interleukin-12 receptor
• UniProt Gene Name: IL12RB1
• UniProt Synonym Gene Names: IL12R; IL12RB; IL-12 receptor subunit beta-1; IL-12R subunit beta-1; IL-12R-beta-1; IL-12RB1
• UniProt Entry Name: I12R1_HUMAN

NCBI Description

The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

IL12RB1: Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade. Defects in IL12RB1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Belongs to the type I cytokine receptor family. Type 2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-12 receptor activity; interleukin-23 binding; interleukin-23 receptor activity; interleukin-12 receptor binding

Biological Process: positive regulation of memory T cell differentiation; positive regulation of interferon-gamma production; peptidyl-tyrosine phosphorylation; positive regulation of T-helper 1 type immune response; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; positive regulation of activated T cell proliferation; signal transduction; positive regulation of defense response to virus by host

Disease: Immunodeficiency 30